拟南芥成熟胚乳解剖及从成熟种子组织中分离基因组DNA
Arabidopsis thaliana Mature Endosperm Dissection and Isolation of Genomic DNA from Mature Seed Tissues.
作者信息
Iwasaki Mayumi, Lopez-Molina Luis
机构信息
Department of Plant Biology, University of Geneva, Geneva, Switzerland.
Institute for Genetics and Genomics in Geneva (iGE3), University of Geneva, Geneva, Switzerland.
出版信息
Methods Mol Biol. 2021;2250:239-243. doi: 10.1007/978-1-0716-1134-0_22.
PMID:33900609
Abstract
We describe methods to separate endosperms and embryos from Arabidopsis thaliana mature seeds in large amounts and to isolate high-quality genomic DNA from those tissues. The resulting materials are suitable for analysis of DNA methylation by bisulfite sequencing or histone modifications by chromatin immunoprecipitation (ChIP).
摘要
我们描述了从拟南芥成熟种子中大量分离胚乳和胚胎的方法，以及从这些组织中分离高质量基因组DNA的方法。所得材料适用于通过亚硫酸氢盐测序分析DNA甲基化或通过染色质免疫沉淀（ChIP）分析组蛋白修饰。
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