Reiner Z, Cvrtila D, Petric V
Institute of Pathophysiology, Medical Faculty, University of Zagreb, University Hospital Rebro, Yugoslavia.
Arch Otorhinolaryngol. 1988;245(1):47-9. doi: 10.1007/BF00463548.
There are many data that suggest that the larynx is a target organ for steroid hormones, especially androgens. Since laryngeal cancers occur much more often in males, it is conceivable that androgens might be the growth-stimulating factor for such tumors. In order to test this concept further, neoplastic tissues from 68 patients (64 males, 4 females) were obtained during planned total laryngectomies and were tested for the presence of androgen (AR) and estrogen (ER) receptors. In 18 cases progesterone receptors (PgR) were also quantified. The dextran-coated charcoal technique was used to determine hormone receptors and the number of the binding sites and the equilibrium constant of dissociation were calculated according to Scatchard. 3H-R1881 was used as the ligand for the measurement of AR, 3H-estradiol for ER, and 3H-R5020 for PgR. AR was present in 11 tumor specimens (1 female, 10 males) and the concentration varied from 2.8 to 17.1 fmol/mg proteins. ER were found in 6 tumors (1 female, 5 males) in concentrations of 2.9-11.2 fmol/mg proteins. Three tumor specimens contained both AR and ER. All tumors analyzed lacked PgR. These results suggest that at least some laryngeal carcinomas might be hormone dependent, indicating that certain patients could benefit from antiandrogen therapy.
有许多数据表明，喉是类固醇激素，尤其是雄激素的靶器官。由于喉癌在男性中更为常见，可以想象雄激素可能是此类肿瘤的生长刺激因子。为了进一步验证这一概念，在计划进行的全喉切除术中获取了68例患者（64例男性，4例女性）的肿瘤组织，并检测了雄激素（AR）和雌激素（ER）受体的存在情况。在18例病例中还对孕激素受体（PgR）进行了定量分析。采用葡聚糖包被活性炭技术测定激素受体，并根据Scatchard法计算结合位点数量和解离平衡常数。使用3H-R1881作为测量AR的配体，3H-雌二醇用于测量ER，3H-R5020用于测量PgR。11个肿瘤标本（1例女性，10例男性）中存在AR，浓度在2.8至17.1 fmol/mg蛋白质之间。在6个肿瘤（1例女性，5例男性）中发现了ER，浓度为2.9 - 11.2 fmol/mg蛋白质。3个肿瘤标本同时含有AR和ER。所有分析的肿瘤均缺乏PgR。这些结果表明，至少某些喉癌可能依赖激素，这表明某些患者可能从抗雄激素治疗中获益。
