Field L M, Devonshire A L, Forde B G
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, U.K.
Biochem J. 1988 Apr 1;251(1):309-12. doi: 10.1042/bj2510309.
cDNA clones for the esterase (E4) responsible for broad insecticide resistance in peach-potato aphids (Myzus persicae Sulz.) were isolated and used to study the molecular basis of resistance. Increased esterase synthesis by resistant aphids was found to be associated with amplification of the structural gene for the esterase (E4 or its closely related variant, FE4), the degree of amplification being correlated with the activity of the esterase and the level of resistance. Hybridization of the cDNA clones to genomic Southern blots showed that only some of the esterase-related restriction fragments are amplified. Qualitative differences between restriction patterns in different clones of resistant aphids correlated with the presence or absence of a specific chromosome translocation and with production of E4 or FE4.
分离出了桃蚜（烟蚜）中负责广泛抗杀虫剂的酯酶（E4）的cDNA克隆，并用于研究抗性的分子基础。发现抗性蚜虫酯酶合成增加与酯酶结构基因（E4或其密切相关变体FE4）的扩增有关，扩增程度与酯酶活性和抗性水平相关。cDNA克隆与基因组Southern印迹杂交表明，只有一些与酯酶相关的限制性片段被扩增。抗性蚜虫不同克隆的限制性图谱的定性差异与特定染色体易位的存在与否以及E4或FE4的产生相关。
