Gschwendt M, Kittstein W, Marks F
German Cancer Research Center, Institute of Biochemistry, Heidelberg.
Biochem Biophys Res Commun. 1988 Jun 30;153(3):1129-35. doi: 10.1016/s0006-291x(88)81345-7.
Topical application of the phorbol ester TPA to mouse skin causes an increase in the amount of elongation factor 2 (EF-2), a factor in eukaryotic protein synthesis, in the epidermal cytosol (2- to 3-fold) and particulate fraction (7-fold). Furthermore, as a consequence of this TPA treatment the activity of an epidermal EF-2 phosphatase is stimulated. The EF-2 phosphatase has an apparent molecular weight of around 38,000 daltons. The enzyme activity is induced as early as 45 minutes after TPA treatment and remains at the elevated level for more than 17 hours. Both of the TPA-induced effects result in an increase in unphosphorylated, i.e. active EF-2 and can be suppressed by cyclosporine A.
将佛波酯十四烷酰佛波醇乙酯（TPA）局部应用于小鼠皮肤，可使真核生物蛋白质合成因子延伸因子2（EF-2）在表皮胞质溶胶中的含量增加（2至3倍），在颗粒部分中的含量增加（7倍）。此外，经TPA处理后，表皮EF-2磷酸酶的活性受到刺激。EF-2磷酸酶的表观分子量约为38,000道尔顿。该酶活性在TPA处理后45分钟就开始诱导，并在17小时以上保持在升高水平。TPA诱导的这两种效应均导致未磷酸化即活性EF-2的增加，并且可被环孢菌素A抑制。
