Department of Biochemistry and Molecular Biology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China; Department of Biochemistry, Faculty of Agriculture, AL-Azhar University, Nasr City, Cairo 11751, Egypt.
Department of Biochemistry and Molecular Biology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Bioorg Chem. 2021 Jul;112:104896. doi: 10.1016/j.bioorg.2021.104896. Epub 2021 Apr 7.
Effective and precise eradication of Helicobacter pylori (H. pylori) is the most promising approach to avoid H. pylori-related gastrointestinal disorders. The present study was conducted to demonstrate the efficacy of the co-delivery of hesperidin (Hesp) and clarithromycin (CLR) in nanostructured lipid carriers (NLCs) against H. pylori. We have produced a new delivery system by combining bioflavonoid Hesp and CLR NLCs to address the failure in single antibiotic therapies. Briefly, a blend of solid lipid, liquid lipid, and surfactant was used. Homogeneous NLCs with all the formulations showed a nano size and surface-negative charge and presented high in vitro stability and slow release of the drug even after 24 h. Bioimaging studies by scanning electron microscopy, transmission electron microscopy, and imaging flow cytometry indicated that NLCs interacted with the membrane by adhering to the outer cell membrane and disrupted the membrane that resulted in the leakage of cytoplasmic contents. The prepared NLCs provide sustained and controlled drug release that can be used to increase the rate of H. pylori eradication.
有效且精准地根除幽门螺杆菌（H. pylori）是避免与 H. pylori 相关的胃肠道疾病的最有前景的方法。本研究旨在展示将橙皮苷（Hesp）和克拉霉素（CLR）共同递送至纳米结构脂质载体（NLCs）中对抗 H. pylori 的功效。我们通过将生物类黄酮 Hesp 和 CLR NLCs 相结合来产生新的递药系统，以解决单一抗生素疗法失败的问题。简要地说，使用了固体脂质、液体脂质和表面活性剂的混合物。所有配方的均一 NLCs 具有纳米尺寸和表面负电荷，并表现出高体外稳定性和药物缓慢释放，即使在 24 小时后也是如此。通过扫描电子显微镜、透射电子显微镜和成像流式细胞术进行的生物成像研究表明，NLCs 通过附着在细胞膜上来与膜相互作用，并破坏导致细胞质内容物泄漏的膜。所制备的 NLCs 提供了持续和控制药物释放，可以用于提高 H. pylori 根除率。
