Department of Pathology, Zhejiang Hospital, Hangzhou, Zhejiang, China.
Department of Geriatrics, Zhejiang Provincial Key Lab of Geriatrics, Hangzhou, Zhejiang, China.
Aging (Albany NY). 2021 Apr 26;13(10):13663-13679. doi: 10.18632/aging.202909.
The function of centromere protein U () gene in breast cancer has not been well understood. Therefore, we explored the expression profiles of gene in breast carcinoma to better understand the functions of this gene, as well as the relationship between expression and the prognosis of breast carcinoma patients. Our results indicate that was expressed at significantly higher levels in cancerous tissues than in normal tissues. Furthermore, expression correlated significantly with many clinicopathological characteristics of breast cancer. In addition, we discovered that high levels of expression predicted poor prognosis in patients with breast cancer. Functional investigation revealed that 180 genes exhibited co-expression with . Functional annotation indicated that 17 of these genes were involved in the PLK1 signaling pathway, with most of them (16/17) being expressed at significantly higher levels in malignant tissues compared with normal controls and correlating with a poor prognosis. Subsequently, we found that four miRNAs, namely hsa-miR-543, hsa-miR-495-3p, hsa-miR-485-3p, and hsa-miR-337-3p, could be regarded as potential expression regulators. Then, five lncRNAs were predicted to potentially bind to the four miRNAs. Combination of the results from expression, survival, correlation analysis and functional experiments analysis demonstrated the link between lncRNA GATA3-AS1/miR-495-3p/CENPU axis and prognosis of breast cancer. In conclusion, CENPU could be involved in cell cycle progression through PLK1 signaling pathway.
中心体蛋白 U () 基因在乳腺癌中的功能尚未得到很好的理解。因此，我们探索了 基因在乳腺癌中的表达谱，以更好地了解该基因的功能，以及 表达与乳腺癌患者预后之间的关系。我们的结果表明，在癌组织中 基因的表达水平明显高于正常组织。此外， 表达与乳腺癌的许多临床病理特征显著相关。此外，我们发现高水平的 表达预示着乳腺癌患者的预后不良。功能研究表明，有 180 个基因与 呈共表达。功能注释表明，这些基因中有 17 个与 PLK1 信号通路有关，其中大多数（16/17）在恶性组织中的表达水平明显高于正常对照，与预后不良相关。随后，我们发现四种 miRNA，即 hsa-miR-543、hsa-miR-495-3p、hsa-miR-485-3p 和 hsa-miR-337-3p，可以作为潜在的 表达调节剂。然后，预测了五个 lncRNA 可能与这四个 miRNA 结合。表达、生存、相关性分析和功能实验分析的结果组合表明，lncRNA GATA3-AS1/miR-495-3p/CENPU 轴与乳腺癌的预后之间存在联系。总之，CENPU 可能通过 PLK1 信号通路参与细胞周期进程。
