Helander K G, Tribukait B
Department of Pathology, University of Umeå, Sweden.
Anal Quant Cytol Histol. 1988 Apr;10(2):127-33.
In a study of the correlation between mean nuclear size and DNA content in urinary bladder carcinoma, the modal DNA values of cell suspensions from 125 biopsies, obtained from 86 patients with malignant or normal urinary bladder epithelium, were analyzed by flow cytometry (FCM). Light microscopic measurements of nuclear size were carried out on smears from the same material. The results were correlated to the histopathologic stage and grade. The mean nuclear volumes were significantly larger in diploid tumor cells than in cells of normal epithelium. Aneuploid tumors showed significantly larger nuclei than did diploid tumors. Although there was a significant correlation between increases in the nuclear volume and in the DNA content, there was some overlapping between various grades of malignancy: mean nuclear volumes in aneuploid grade 2 tumors did not differ from those in aneuploid grade 3 tumors. A combination of FCM and morphometry discriminated all but 16% of the tumors from the normal cases. It is concluded that FCM and morphometry are complementary and can be used for the objective characterization of urinary bladder carcinomas.
在一项关于膀胱癌细胞核平均大小与DNA含量相关性的研究中，采用流式细胞术（FCM）分析了来自86例恶性或正常膀胱上皮患者的125份活检组织细胞悬液的众数DNA值。对相同材料涂片进行细胞核大小的光学显微镜测量。结果与组织病理学分期和分级相关。二倍体肿瘤细胞的平均核体积明显大于正常上皮细胞。非整倍体肿瘤的细胞核明显大于二倍体肿瘤。虽然核体积增加与DNA含量增加之间存在显著相关性，但不同恶性程度之间存在一定重叠：非整倍体2级肿瘤的平均核体积与非整倍体3级肿瘤的平均核体积没有差异。流式细胞术和形态计量学相结合能将除16%的肿瘤外的所有肿瘤与正常病例区分开来。得出的结论是，流式细胞术和形态计量学具有互补性，可用于膀胱癌的客观特征描述。
