Local and CNS-Wide Astrocyte Intracellular Calcium Signaling Attenuation with CalEx Mice.

Astrocytes exist throughout the CNS and affect neural circuits and behavior through intracellular Ca signaling. Studying the function(s) of astrocyte Ca signaling has proven difficult because of the paucity of tools to achieve selective attenuation. Based on recent studies, we generated and used male and female knock-in mice for Cre-dependent expression of mCherry-tagged hPMCA2w/b to attenuate astrocyte Ca signaling in genetically defined cells (CalEx mice for lcium trusion). We characterized CalEx mice following local AAV-Cre microinjections into the striatum and found reduced astrocyte Ca signaling (∼90%) accompanied with repetitive self-grooming behavior. We also crossed CalEx mice to astrocyte-specific -Cre/ERT2 mice to achieve inducible global CNS-wide Ca signaling attenuation. Within 6 d of induction in the bigenic mice, we observed significantly altered ambulation in the open field, disrupted motor coordination and gait, and premature lethality. Furthermore, with histologic, imaging, and transcriptomic analyses, we identified cellular and molecular alterations in the cerebellum following mCherry-tagged hPMCA2w/b expression. Our data show that expression of mCherry-tagged hPMCA2w/b with CalEx mice throughout the CNS resulted in substantial attenuation of astrocyte Ca signaling and significant behavioral alterations in adult mice. We interpreted these findings candidly in relation to the ability of CalEx to attenuate astrocyte Ca signaling, with regards to additional mechanistic interpretations of the data, and their relation to past studies that reduced astrocyte Ca signaling throughout the CNS. The data and resources provide complementary ways to interrogate the function(s) of astrocytes in multiple experimental scenarios. Astrocytes represent a significant fraction of all brain cells and tile the entire central nervous system. Unlike neurons, astrocytes lack propagated electrical signals. Instead, astrocytes are proposed to use diverse and dynamic intracellular Ca signals to communicate with other cells. An open question concerns if and how astrocyte Ca signaling regulates behavior in adult mice. We approached this problem by generating a new transgenic mouse line to achieve inducible astrocyte Ca signaling attenuation We report our data with this mouse line and we interpret the findings candidly in relation to past studies and within the framework of different mechanistic interpretations.