Laboratorio de Patología Vegetal, Instituto de Investigaciones Agropecuarias y Forestales (IIAF), Universidad Michoacana de San Nicolás de Hidalgo, Morelia-Zinapécuaro, Michoacán 58880, México.
Mycotoxin Prevention and Applied Microbiology Research Unit, U.S. Department of Agriculture Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria 61604, U.S.A.
Plant Dis. 2021 Oct;105(10):2822-2829. doi: 10.1094/PDIS-09-20-1942-RE. Epub 2021 Nov 9.
(rosy trumpet) is an economically important neotropical tree in Mexico that is highly valued for the quality of its wood, which is used for furniture, crafts, and packing, and for its use as an ornamental and shade tree in parks and gardens. During surveys conducted in the lower Balsas River Basin region in the states of Guerrero and Michoacán, symptoms of floral malformation were detected in trees. The main objectives of this study were to describe this new disease, to determine its causal agent, and to identify it using DNA sequence data. A second set of objectives was to analyze the phylogenetic relationship of the causal agent to spp. associated with trees with malformation surveyed in the same region and to compare mycotoxin production and the mating type idiomorphs of fusaria recovered from and . showed malformed inflorescences with multiple tightly curled shoots and shortened internodes. A total of 31 isolates recovered from symptomatic ( = 20) and ( = 11) trees were identified by molecular analysis as . Pathogenicity tests showed that isolates of recovered from induced malformation in inoculated seedlings. Eighteen isolates were tested for their ability to produce mycotoxins and other secondary metabolites. Moniliformin, fusaric acid, bikaverin, beauvericin, aurofusarin. and 8-O-methylbostrycoidin were produced by at least one strain of the 18 isolates tested. A multiplex PCR assay for mating type idiomorph revealed that 22 isolates were and that 9 were . Here, we report a new disease of in Mexico caused by .
（rosy trumpet）是墨西哥一种具有经济重要性的新热带树种，其木材质量优良，用于制作家具、工艺品和包装，也因其作为公园和花园的观赏和遮荫树而受到重视。在格雷罗州和米却肯州的下巴尔萨斯河流域进行的调查中，发现了树木出现花朵畸形的症状。本研究的主要目的是描述这种新病害，确定其病原物，并利用 DNA 序列数据对其进行鉴定。另一组目标是分析病原物与在同一地区调查的与畸形树木相关的 spp.的系统发育关系，并比较从 和 中回收的蕈菌的产毒素和交配型异型现象。表现出畸形花序，有多根紧密卷曲的嫩枝和缩短的节间。从有症状的 （ = 20）和 （ = 11）树木中总共回收了 31 个分离株，通过分子分析鉴定为 。致病性试验表明，从 中回收的 分离株在接种的 幼苗中引起畸形。对 18 个分离株进行了产毒素和其他次生代谢物的能力测试。至少有 18 个分离株产生了单端孢霉烯、蕈酸、比卡可汀、 beauvericin、aurofusarin 和 8-O-甲基博斯苦霉素。对交配型异型现象的多重 PCR 检测表明，22 个分离株为 ，9 个为 。在这里，我们报告了墨西哥一种由 引起的 的新病害。
