University of Gondar Comprehensive Specializes Hospital, University of Gondar, Gondar, Ethiopia.
Department of Immunology and Molecular Biology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
J Evid Based Integr Med. 2021 Jan-Dec;26:2515690X211006344. doi: 10.1177/2515690X211006344.
has become the leading cause of morbidity and death in humans worldwide. Thus, genetic variability of the host plays a major role in human susceptibility to the pathogen, among others. Therefore, the objective of this finding was to assess the association of genetic polymorphisms of cytokines with tuberculosis infection.
A cross-sectional study was conducted between January and May 2018. Five ml of whole blood was collected and extracted the genomic DNA through simple salting out method. The patterns of genetic polymorphism were determined by amplification refractory method PCR using specific primers. Finally, the PCR run on electrophoresis of agarose gel and the band was visualized under UV light. A logistical regression model has been adapted to assess the association of genetic polymorphisms with tubercular infection. In order to determine the association between the explanatory and outcome variable, the odds ratio with 95% CI was calculated. P < 0.05 is a statistically significant value.
In present study, the frequency of TNF-α -308 G allele and GG genotype OR (95% CI)= 0.20 (0.11-0.37), and OR (95% CI)= 0.29 (0.18-0.46)), respectively) and IFN-γ +874 A allele and AA genotype OR (95% CI)= 3.80 (2.11-6.86) and (OR (95% CI)= 1.61(1.13-2.28), respectively) were significantly associated with tuberculosis incidence. In contrast, there is no significant correlation between IL-10 -1082 A and AA of allele and genotype, respectively in tuberculosis patients (p > 0.05) was evident.
From our finding, the genetic variability of TNF-α -308 A and IFN-γ +874 alleles are the potent host genetic risk factors associated with tuberculosis infection.
结核病已成为全球范围内导致发病率和死亡率的主要原因。因此，宿主的遗传变异性在人类对病原体的易感性中起着重要作用。因此，本研究旨在评估细胞因子的遗传多态性与结核病感染之间的关联。
本研究为 2018 年 1 月至 5 月进行的一项横断面研究。采集 5ml 全血，通过简单盐析法提取基因组 DNA。通过使用特异性引物的扩增抗性方法 PCR 确定遗传多态性模式。最后，在琼脂糖凝胶电泳上运行 PCR，在紫外光下观察条带。采用逻辑回归模型评估遗传多态性与结核感染的关联。为了确定解释变量和结果变量之间的关联，计算了比值比及其 95%置信区间。P<0.05 为统计学显著值。
在本研究中，TNF-α-308 G 等位基因和 GG 基因型的 OR（95%CI）分别为 0.20（0.11-0.37）和 0.29（0.18-0.46），IFN-γ+874 A 等位基因和 AA 基因型的 OR（95%CI）分别为 3.80（2.11-6.86）和 1.61（1.13-2.28），与结核病的发病显著相关。相比之下，IL-10-1082 A 等位基因和 AA 基因型与结核病患者无显著相关性（p>0.05）。
根据我们的发现，TNF-α-308 A 和 IFN-γ+874 等位基因的遗传变异性是与结核病感染相关的潜在宿主遗传危险因素。
