Akhrem A A, Romanov S L, Karasev V P, Kisel' M A, Kiselev P A
Biofizika. 1988 Mar-Apr;33(2):275-81.
Studies were carried out of temperature quenching of self-fluorescence of cytochrome P-450 in solution and liposomes from natural phosphatidylcholine, dimiristoylphosphatidylcholine, dipalmitoylphosphatidylcholine. The fluorescence spectrum of cytochrome P-450 is a superposition of triptophane and tyrosine components. During protein incorporation into liposomes a significant short-wave shift of the emission spectrum takes place. Temperature dependence of the intensity of cytochrome P-450 self-fluorescence in solution has bends at 30, 45 and 50 degrees C. When protein is incorporated into liposomes the location of bends depends on individual properties of lipids forming the bilayer. Effect of lipid surrounding on temperature conformational rearrangements of cytochrome P-450 molecule is discussed.
对溶液中以及天然磷脂酰胆碱、二肉豆蔻酰磷脂酰胆碱、二棕榈酰磷脂酰胆碱脂质体中细胞色素P - 450自身荧光的温度猝灭进行了研究。细胞色素P - 450的荧光光谱是色氨酸和酪氨酸成分的叠加。在蛋白质掺入脂质体的过程中，发射光谱发生了显著的短波位移。溶液中细胞色素P - 450自身荧光强度的温度依赖性在30、45和50摄氏度处出现转折。当蛋白质掺入脂质体时，转折的位置取决于形成双层的脂质的个体特性。讨论了脂质环境对细胞色素P - 450分子温度构象重排的影响。
