[Distribution of the heavy isotope of carbon (13C) in biological systems].

Causes conditioning fractionation of carbon isotopes in biological systems are considered. Concepts of E. M. Galimov are discussed. According to these concepts distribution of carbon isotopes between biomolecules and their fragments is quaziequilibrium, i. e. it differs from the equilibrium one by a constant multiplier, which is the same for all the biomolecules of an organism but different for various organisms. These concepts have no theoretical grounds and do not agree with the experimental evidence available. An analysis of experimental data, as well as theoretical considerations, indicate that the observed differences in isotope composition of metabolytes and their fragments in the living organisms are conditioned by the kinetic isotope effects of carbon at the stages of their enzymic transformations and by the portion of substances participating in the reaction. It means that these differences do not depend directly on the constants characterizing the equilibrium distribution of carbon isotopes between corresponding compounds and between different groups inside their molecules.