（一氧化碳）肌红蛋白光解反应的时间分辨圆二色性和吸收研究
Time-resolved circular dichroism and absorption studies of the photolysis reaction of (carbonmonoxy)myoglobin.
作者信息
Milder S J, Bjorling S C, Kuntz I D, Kliger D S
机构信息
Department of Chemistry, University of California, Santa Cruz 95064.
出版信息
Biophys J. 1988 May;53(5):659-64. doi: 10.1016/S0006-3495(88)83146-1.
Abstract
Time-resolved circular dichroism (TRCD) and absorption spectroscopy are used to follow the photolysis reaction of (carbonmonoxy)myoglobin (MbCO). Following the spectral changes associated with the initial loss of CO, a subtle change is observed in the visible absorption spectrum of the Mb product on a time scale of a few hundred nanoseconds. No changes are seen in the CD spectrum of Mb in the visible and near-UV regions subsequent to the loss of CO. The data suggest the existence of an intermediate found after ligand loss from MbCO that is similar in structure to the final Mb product.
摘要
时间分辨圆二色光谱（TRCD）和吸收光谱被用于跟踪（一氧化碳）肌红蛋白（MbCO）的光解反应。在跟踪与CO初始损失相关的光谱变化时，在几百纳秒的时间尺度上，观察到Mb产物的可见吸收光谱有细微变化。CO损失后，Mb在可见光和近紫外区域的圆二色光谱没有变化。数据表明，在MbCO失去配体后存在一种中间体，其结构与最终的Mb产物相似。
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