Hall R M, Mattick J S, Nagley P, Cobon G S, Eastwood F W, Linnane A W
Mol Biol Rep. 1977 Dec;3(6):443-9. doi: 10.1007/BF00808386.
We have studied the effects on the yeast mitochondrial genome of four analogues of ethidium bromide, in which the phenyl moieyt has been replaced by linear alkyl chains of lengths varying from seven to fifteen carbon atoms. These analogues are more efficient than ethidium bromide in inducing petite mutants in Saccharomyces cervisiae. The drugs also cause a loss of mtDNA from the cells in vivo; however these analogues are in fact less effective inhibitors of mitochondrial DNA replication per se, as shown by direct in vitro studies. It is concluded that these analogues are more efficient than ethidium bromide in causing the fragmentation of mitochondrial DNA in S. cervisiae.
我们研究了四种溴化乙锭类似物对酵母线粒体基因组的影响，其中苯基部分已被长度从7到15个碳原子不等的直链烷基链取代。这些类似物在诱导酿酒酵母中的小菌落突变体方面比溴化乙锭更有效。这些药物在体内也会导致细胞中mtDNA的丢失；然而，如直接的体外研究所表明的，这些类似物实际上本身作为线粒体DNA复制的抑制剂效果较差。得出的结论是，这些类似物在导致酿酒酵母线粒体DNA断裂方面比溴化乙锭更有效。
