Foucrier J, Pechinot D, Rigaut J P, Feldmann G
Laboratoire de Biologie Cellulaire, INSERM U24, UFR Xavier Bichat, Université Paris, France.
Biol Cell. 1988;62(2):125-31.
In a previous work it was shown that transferrin (Tf) secretion is directly related to the membrane surface area of hepatocytes (Péchinot D. et al. [31]). The aim of the present work was to search for a possible relationship between Tf secretion and hepatocytic ploidy using a semi-automatic image analysis method. A determination of Tf secretion by isolated normal adult hepatocytes was achieved at the single cell level, using a modified reverse hemolytic plaque test. A Feulgen reaction was also performed on these hepatocytes. It allowed the evaluation, for each secreting hepatocyte, of the quantity of Tf secreted and its nuclear characteristics. Discrimination between diploid (2c) and tetraploid (4c and 2c2c) hepatocytes was performed and the amount of Tf secreted by each subpopulation determined. It appeared that a 2-fold secretion ratio was not found between tetraploid and diploid hepatocytes. These results suggest, as Tf production is not directly proportional to the degree of ploidy of hepatocytes, that some not yet elucidated regulatory mechanisms may act on Tf gene expression.
在之前的一项研究中发现，转铁蛋白（Tf）的分泌与肝细胞的膜表面积直接相关（佩希诺特D.等人[31]）。本研究的目的是使用半自动图像分析方法寻找Tf分泌与肝细胞倍性之间可能存在的关系。使用改良的反向溶血空斑试验，在单细胞水平上对分离的正常成年肝细胞的Tf分泌进行了测定。还对这些肝细胞进行了福尔根反应。这使得能够评估每个分泌性肝细胞分泌的Tf量及其核特征。对二倍体（2c）和四倍体（4c和2c2c）肝细胞进行了区分，并确定了每个亚群分泌的Tf量。结果表明，四倍体和二倍体肝细胞之间未发现2倍的分泌率。这些结果表明，由于Tf的产生与肝细胞的倍性程度不成正比，可能存在一些尚未阐明的调节机制作用于Tf基因表达。
