National Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
Plant Signal Behav. 2021 Jun 3;16(6):1913301. doi: 10.1080/15592324.2021.1913301. Epub 2021 Apr 27.
Plant genomes encode numerous proteins with obscure features (POFs) that lack recognized domains or motifs. However, there is little functional information for POFs even in because biochemical, physiological, and genetic assay are required for the functional annotations of POFs. Here, we identified a small gene family, the endoplasmic reticulum-localized POF (ERP) family, in . Phylogenetic analysis revealed that the number of ERP family members was conserved in the plant kingdom, suggesting strong selective pressure was imposed on ERP family during plant evolution. No recognizable domains were identified in the predicted ERP proteins, except for the N-terminal signal peptide. ERPs were found to be widely expressed during development and showed endoplasmic reticulum localization. It was reported that is an inositol-1,4,5-trisphosphate 5-phosphatase (5PTase), but could not substitute for in precocious pollen germination, indicating that did not have the similar functions as in inositol 1,4,5-trisphosphate [Ins(1,4,5)] metabolism. Further studies are needed to dissect the functions of ERP family proteins in development.
植物基因组编码了许多具有模糊特征（POF）的蛋白质，这些蛋白质缺乏公认的结构域或基序。然而，即使在 中，也几乎没有关于 POF 的功能信息，因为需要进行生化、生理和遗传测定来对 POF 进行功能注释。在这里，我们在 中鉴定了一个小的基因家族，内质网定位的 POF（ERP）家族。系统发育分析表明，ERP 家族成员的数量在植物界是保守的，这表明在植物进化过程中，ERP 家族受到了强烈的选择压力。除了 N 端信号肽外，预测的 ERP 蛋白中没有发现可识别的结构域。ERP 蛋白在 发育过程中广泛表达，并表现出内质网定位。有报道称 是肌醇 1,4,5-三磷酸 5-磷酸酶（5PTase），但 不能替代 在花粉提前萌发中的作用，表明 在肌醇 1,4,5-三磷酸 [Ins(1,4,5)]代谢中没有与 相似的功能。需要进一步研究来剖析 ERP 家族蛋白在 发育中的功能。
