Li Deming, Wang Ze, Zhang Chunyan, Xu Cunshuan
State Key Laboratory Cell Differentiation and Regulation, Henan Normal University, Xinxiang, Henan China.
Henan International Joint Laboratory of Pulmonary Fibrosis, Henan Normal University, Xinxiang, Henan China.
Turk J Biol. 2021 Apr 20;45(2):225-234. doi: 10.3906/biy-2010-51. eCollection 2021.
Inflammation has a dual effect: it can protect the body and destroy tissue and cell as well. The purpose of this experiment was to determine the role of in liver regeneration (LR) after partial hepatectomy (PH) in aged mice. The wild-type (WT, n = 36) and the knockout (KO, n = 36) 24-month-old C57BL/6J mice underwent two-thirds PH; 33 WT mice underwent sham operation. Liver coefficient was calculated by liver/body weight. The mRNA and protein expressions of genes were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting methods, respectively. Compared with WT mice, liver coefficient was lower in the KO aged mice at 168 and 192 h (p = 0.039 and p = 0.027). The mRNA transcription of inflammation-related genes and cell cycle-associated genes decreased or delayed. The protein expressions of proliferation-related marker PCNA and proliferation-associated signaling pathway components JNK1, NF-κB and STAT3 reduced or retarded. There was stronger activation of proapoptotic proteins caspase-3, caspase-8 and BAX in the KO mice at different time points (p < 0.05 or p < 0.01). KO reduced inflammation and caused impaired liver regeneration after 2/3 partial hepatectomy in aged mice. Maintaining proper inflammation may contribute to regeneration after liver partly surgical resection in the elderly.
它既能保护身体，也能破坏组织和细胞。本实验的目的是确定[具体物质]在老年小鼠部分肝切除术后肝脏再生（LR）中的作用。24月龄的野生型（WT，n = 36）和[具体物质]基因敲除（KO，n = 36）C57BL/6J小鼠接受了三分之二肝切除术；33只WT小鼠接受了假手术。肝脏系数通过肝脏重量/体重计算得出。分别采用定量实时聚合酶链反应（qRT-PCR）和蛋白质印迹法评估基因的mRNA和蛋白质表达。与WT小鼠相比，[具体物质]基因敲除的老年小鼠在168小时和192小时时肝脏系数较低（p = 0.039和p = 0.027）。炎症相关基因和细胞周期相关基因的mRNA转录减少或延迟。增殖相关标志物PCNA以及增殖相关信号通路成分JNK1、NF-κB和STAT3的蛋白质表达降低或延迟。在不同时间点，[具体物质]基因敲除小鼠中促凋亡蛋白caspase-3、caspase-8和BAX的激活更强（p < 0.05或p < 0.01）。[具体物质]基因敲除减少了炎症，并导致老年小鼠三分之二部分肝切除术后肝脏再生受损。维持适当的炎症可能有助于老年人肝脏部分手术切除后的再生。
