Amini Nooshin, Shiravi Abdolhossein, Mirazi Naser, Hojati Vida, Abbasalipourkabir Roghayeh
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Department of Biology, Faculty of Basic Sciences, Bu- Ali Sina University, Hamedan, Iran.
Avicenna J Phytomed. 2021 Mar-Apr;11(2):199-209.
Protective effects of raspberry ( L.) fruit extract on pituitary-gonadal axis and testicular tissue in diabetic male rats, were investigated.
Sixty male rats were divided into control, sham (saline treated), streptozotocin (STZ)-diabetic, and STZ-diabetic animals treated with 50, 100 and 200 mg/kg/day of raspberry extract. After 4 weeks, blood samples were obtained and left testes were removed and prepared for histopathological studies. Serum levels of Luteinizing hormone (LH), Follicle stimulating hormone (FSH), testosterone, Nitric oxide (NO), and malondialdehyde (MDA), as well as superoxide dismutase (SOD) and catalase (CAT) activity level were assayed. Sperm number and motility in the epididymis samples were measured. Data were analyzed using ANOVA (one-way analysis of variance).
Serum levels of LH, FSH and MDA significantly increased in diabetic rats, however, treatment with the extract significantly reversed the alterations. Serum levels of testosterone and NO, activity of SOD and CAT, and sperm number and motility significantly decreased and severe destruction of testicular histology was observed in diabetic animals while treatment with the extract significantly reversed the pathologic alterations observed in diabetic rats. According to the results, 100 and 200 mg/kg of the extract were able to effectively reverse the diabetes complications.
Our findings demonstrated that the fruit extract of raspberry has protective effects on male reproductive system in diabetic rats partially due to its improving effects on NO system, and SOD and CAT activity.
研究树莓果实提取物对糖尿病雄性大鼠垂体 - 性腺轴及睾丸组织的保护作用。
将60只雄性大鼠分为对照组、假手术组（生理盐水处理）、链脲佐菌素（STZ）糖尿病组，以及分别用50、100和200mg/kg/天树莓提取物处理的STZ糖尿病组动物。4周后，采集血样，摘除左侧睾丸并制备用于组织病理学研究。检测血清促黄体生成素（LH）、促卵泡生成素（FSH）、睾酮、一氧化氮（NO）和丙二醛（MDA）水平，以及超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性水平。测量附睾样本中的精子数量和活力。数据采用方差分析（单向方差分析）进行分析。
糖尿病大鼠血清LH、FSH和MDA水平显著升高，但提取物处理可显著逆转这些变化。糖尿病动物血清睾酮和NO水平、SOD和CAT活性以及精子数量和活力显著降低，且观察到睾丸组织严重破坏，而提取物处理可显著逆转糖尿病大鼠中观察到的病理变化。根据结果，100和200mg/kg的提取物能够有效逆转糖尿病并发症。
我们的研究结果表明，树莓果实提取物对糖尿病大鼠的雄性生殖系统具有保护作用，部分原因是其对NO系统以及SOD和CAT活性的改善作用。
