线粒体柠檬酸-草酰乙酸载体在产油真菌白地霉脂肪积累中的作用。
Role of the mitochondrial citrate-oxoglutarate carrier in lipid accumulation in the oleaginous fungus Mortierella alpina.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China.
出版信息
Biotechnol Lett. 2021 Jul;43(7):1455-1466. doi: 10.1007/s10529-021-03133-x. Epub 2021 Apr 27.
OBJECTIVES
The transport of citrate from the mitochondria to the cytoplasm is essential during lipid accumulation. This study aimed to explore the role of mitochondrial citrate-oxoglutarate carrier in lipid accumulation in the oleaginous fungus Mortierella alpina.
RESULTS
Homologous MaYHM (the gene encoding the mitochondrial citrate-oxoglutarate carrier) was overexpressed in M. alpina. The fatty acid content of MaYHM-overexpressing recombinant strains was increased by up to 30% compared with the control. Moreover, the intracellular α-ketoglutarate level in recombinant strains was increased by 2.2 fold, together with a 23-35% decrease in NAD-isocitrate dehydrogenase activity compared with the control. The overexpression of MaYHM altered the metabolic flux in the glutamate dehydrogenase shunt and 4-aminobutyric acid shunt during metabolic reprogramming, supplying more carbon to synthesize fatty acids.
CONCLUSIONS
Overexpression of MaYHM resulted in more efflux of citrate from mitochondria to the cytoplasm and enhanced lipid accumulation. These findings provide new perspectives for the improvement of industrial lipid production in M. alpina.
目的
在线粒体中柠檬酸向细胞质的转运对脂质积累至关重要。本研究旨在探索线粒体柠檬酸-草酰乙酸载体在产油真菌白僵菌脂质积累中的作用。
结果
过表达白僵菌同源 MaYHM（编码线粒体柠檬酸-草酰乙酸载体的基因）。与对照相比，MaYHM 过表达重组菌株的脂肪酸含量最高增加了 30%。此外，重组菌株的细胞内α-酮戊二酸水平增加了 2.2 倍，与对照相比，NAD-异柠檬酸脱氢酶活性降低了 23-35%。MaYHM 的过表达改变了谷氨酸脱氢酶分支和 4-氨基丁酸分支在代谢重编程过程中的代谢通量，为合成脂肪酸提供了更多的碳源。
结论
MaYHM 的过表达导致更多的柠檬酸从线粒体向细胞质流出，从而增强了脂质的积累。这些发现为提高白僵菌工业产油提供了新的视角。
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