Symphogen A/S, Ballerup, Denmark.
Methods Mol Biol. 2021;2271:23-45. doi: 10.1007/978-1-0716-1241-5_2.
Intact mass analysis of proteins is simple, fast, and specific, and it effectively provides structural insight into the proteoforms or variants of the analyzed protein. For instance, the multiple glycoforms of recombinant monoclonal antibodies can be effectively analyzed by intact mass spectrometry (MS). A recent development in the Orbitrap technology has made this platform particularly well suited for analysis of large intact biomolecules, and here we describe procedures for performing intact mass analysis of intact glycoproteins using the Orbitrap platform, with the aim of identifying and quantitating the glycoforms. Emphasis is placed on the analysis of biopharmaceutical immunoglobulins (IgGs), but the procedures can be extended to other glycoproteins as needed.
蛋白质的完整质量分析简单、快速且具有特异性，可有效提供分析蛋白质的蛋白质形式或变体的结构见解。例如，完整质谱 (MS) 可有效分析重组单克隆抗体的多种糖型。Orbitrap 技术的最新发展使得该平台特别适合分析大型完整生物分子，在此我们描述了使用 Orbitrap 平台进行完整糖蛋白完整质量分析的程序，目的是鉴定和定量糖型。重点是分析生物制药免疫球蛋白 (IgG)，但根据需要，这些程序也可以扩展到其他糖蛋白。
