Quality Assistance SA, Technoparc de Thudinie 2, Donstiennes, Belgium.
University of Bordeaux, INSERM and CNRS, Laboratoires Acides Nucléiques: Régulations Naturelle et Artificielle (ARNA, U1212, UMR5320), IECB, Pessac, France.
Methods Mol Biol. 2021;2271:179-188. doi: 10.1007/978-1-0716-1241-5_13.
N-glycans are described to have a large influence on the properties of therapeutic proteins, including safety and efficacy. For this reason, the extent and type of glycosylation is a characterization parameter for the analysis of antibodies and other therapeutic proteins. The method described here is a fast and high-throughput method for identification and semiquantification of N-glycans by HILIC-FLR-ESI-MS. Sample preparation has been optimized and simultaneous preparation of a large number of samples can be achieved within a day. The use of MS coupled to fluorescence detection is an additional tool for identifying the N-glycan type.
N-糖链被描述为对治疗性蛋白质的性质有很大影响，包括安全性和疗效。因此，糖基化的程度和类型是分析抗体和其他治疗性蛋白质的特征参数。本文所描述的方法是一种通过亲水作用色谱-荧光检测-电喷雾离子化-质谱（HILIC-FLR-ESI-MS）快速高通量鉴定和半定量分析 N-糖链的方法。已经对样品制备进行了优化，可以在一天内同时制备大量样品。将 MS 与荧光检测相结合是鉴定 N-糖链类型的另一种工具。
