Ghoshal A, Roomi M W, Ahluwalia M, Simmonds W, Rushmore T H, Farber E, Ghoshal A K
Departments of Pathology, University of Toronto, Ontario, Canada.
Cancer Lett. 1988 Jul;41(1):53-62. doi: 10.1016/0304-3835(88)90054-7.
Fischer F-344 male rats, fed a choline-devoid diet that leads to a highly reproducible sequence of biochemical and biological changes with an ultimate development of hepatocellular carcinoma, show elevated levels of glutathione in the liver at 3, 6 and 8 days. Several enzymes related to the metabolism of free radicals, including superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and DT-diaphorase show neither increased nor decreased activity as measured between 12 h and 8 days on the diet. Thus, of several known cellular components related to the possible scavenger of free radicals in the liver, only glutathione responded to the feeding of the CD diet. It is tentatively concluded that a decrease in the levels of possible scavengers for free radicals is not a major basis for the nuclear and mitochondrial lipid peroxidation seen early in rats fed a choline-devoid diet.
费舍尔F-344雄性大鼠食用缺乏胆碱的饮食，这种饮食会导致一系列高度可重复的生化和生物学变化，并最终发展为肝细胞癌。在第3、6和8天时，这些大鼠肝脏中的谷胱甘肽水平升高。几种与自由基代谢相关的酶，包括超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽S-转移酶和DT-黄递酶，在饮食12小时至8天期间，其活性既未增加也未降低。因此，在肝脏中几种已知的与可能的自由基清除剂相关的细胞成分中，只有谷胱甘肽对缺乏胆碱饮食的喂养有反应。初步得出结论，自由基可能清除剂水平的降低不是喂食缺乏胆碱饮食的大鼠早期出现的细胞核和线粒体脂质过氧化的主要原因。
