Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
Chem Commun (Camb). 2021 May 25;57(42):5179-5182. doi: 10.1039/d1cc01106e. Epub 2021 Apr 27.
Herein, a novel co-catalytic ferrocene/hemin/G-quadruplexes/FeO nanoparticles (Fc/HGQs/FeO) nanocomposite was synthesized to significantly magnify the electrochemical signal of ferrocene (Fc) using the synergistic catalysis of hemin/G-quadruplexes (HGQs) and FeO nanoparticles as hydrogen peroxide enzyme mimics for the construction of ultrasensitive electrochemical biosensors. The fabricated electrochemical biosensor can achieve ultrasensitive detection of miRNA-155 ranging from 0.1 fM to 1 nM, as well as a limit of detection of 74.8 aM. This strategy provides a new route to exploring efficient signal labels for signal amplification and provides an impetus to find novel methods for the construction of biosensors for biological detection and the early clinic diagnosis of diseases.
在此，合成了一种新型的共催化二茂铁/血红素/G-四链体/FeO 纳米粒子（Fc/HGQs/FeO）纳米复合材料，利用血红素/G-四链体（HGQs）和 FeO 纳米粒子的协同催化作用，显著放大了二茂铁（Fc）的电化学信号，作为过氧化氢酶模拟物，用于构建超灵敏电化学生物传感器。所构建的电化学生物传感器可实现对 miRNA-155 的超灵敏检测，检测范围从 0.1 fM 到 1 nM，检测限低至 74.8 aM。该策略为探索有效的信号标记物以实现信号放大提供了新途径，并为寻找用于生物检测和疾病早期临床诊断的生物传感器的新型构建方法提供了动力。
