Ludány A, Kellermayer M
Department of Clinical Chemistry, Medical University Pécs, Hungary.
Clin Biochem. 1988 Apr;21(2):107-10. doi: 10.1016/s0009-9120(88)80097-3.
The platelets and leukocytes of human peripheral blood were separated and cultured for 24 h in the same medium. The culture medium was completed with S-methionine. The overall protein synthetic activity of the platelets, compared with leukocytes, was studied with a two-dimensional gel electrophoresis technique combined with fluorographic detection of labelled proteins. Our findings confirm the platelet-origin of a few newly synthesized proteins. The most pronounced newly synthesized platelet protein with a molecular weight of approximately 35 Kda and with a more acidic isoelectric point than that of actin was identified as a cytoskeleton-associated protein.
将人外周血中的血小板和白细胞分离出来，在相同培养基中培养24小时。培养基中添加了S-甲硫氨酸。采用二维凝胶电泳技术结合标记蛋白的荧光检测法，研究了血小板与白细胞相比的整体蛋白质合成活性。我们的研究结果证实了一些新合成蛋白质的血小板来源。一种分子量约为35 kDa且比肌动蛋白具有更酸性等电点的最显著的新合成血小板蛋白被鉴定为一种细胞骨架相关蛋白。
