Varga Z, Kovács E M, Paragh G, Jacob M P, Robert L, Fülöp T
First Department of Medicine, University Medical School, Debrecen, Hungary.
Clin Biochem. 1988 Apr;21(2):127-30. doi: 10.1016/s0009-9120(88)80101-2.
The effect of elastin peptides (kappa-elastin, KE) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) on cytosolic free calcium in polymorphonuclear leukocytes (PMNLs) of healthy middle-aged (35-45 years) and elderly (greater than 60 years) patients with normal and high serum cholesterol level was investigated. The cytosolic free calcium [( Ca2+]i) elevation after stimulation with these compounds was decreased in PMNLs of the aged groups compared to the healthy middle-aged group. The guanine nucleotide binding regulatory Gi protein inhibitor, pertussis toxin (PT) prevented the enhancing effect of KE and FMLP on PMNL free calcium of healthy middle-aged subjects, but could not completely abolish the [Ca2+]i elevation in PMNLs of aged subjects.
研究了弹性蛋白肽（κ-弹性蛋白，KE）和N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）对血清胆固醇水平正常和偏高的健康中年（35 - 45岁）及老年（大于60岁）患者多形核白细胞（PMNLs）胞浆游离钙的影响。与健康中年组相比，老年组PMNLs经这些化合物刺激后胞浆游离钙[（Ca2+]i）升高幅度降低。鸟嘌呤核苷酸结合调节蛋白Gi抑制剂百日咳毒素（PT）可抑制KE和FMLP对健康中年受试者PMNL游离钙的增强作用，但不能完全消除老年受试者PMNLs中[Ca2+]i的升高。
