Vogt W, Welsch I
Institut für Klinische Chemie und Laboratoriumsmedizin, Deutsches Herzzentrum München des Freistaates Bayern, F.R.G.
Clin Chem. 1988 Jul;34(7):1459-61.
A recently introduced fluorescence polarization immunoassay (FPIA) for determination of cyclosporine A in serum and plasma is discussed with regard to its use for whole-blood samples, with and without hemolysis before the assay. The performance characteristics of the modified method are highly satisfactory (within-run CVs 2.06 to 5.50% and 1.99 to 3.39%, respectively; long-term between-run CVs under routine conditions 5.73 to 8.95%). The limit of detection is 30 micrograms/L. Results agree well with those obtained with the RIAs compared, but the modified FPIA is more convenient and faster.
本文讨论了一种最近推出的用于测定血清和血浆中环孢素A的荧光偏振免疫分析方法（FPIA），该方法可用于全血样本，且在分析前可进行溶血或不溶血处理。改进方法的性能特征非常令人满意（批内变异系数分别为2.06%至5.50%和1.99%至3.39%；常规条件下的长期批间变异系数为5.73%至8.95%）。检测限为30微克/升。与所比较的放射免疫分析（RIA）结果吻合良好，但改进后的FPIA更方便、更快。
