盐依赖性高血压大鼠品系肾上腺组织中18-羟脱氧皮质酮和皮质酮的生物合成

Biosynthesis of 18-hydroxydeoxycorticosterone and corticosterone in adrenal tissue of rat strains with salt-dependent hypertension.

作者信息

Wohlfeil S, Neuser D, Morich F J

机构信息

Pharma-Forschungszentrum, BAYER AG, Wuppertal, F.R.G.

出版信息

Clin Exp Hypertens A. 1988;10(4):617-27. doi: 10.3109/10641968809033914.

DOI:10.3109/10641968809033914

PMID:3390964

Abstract

Biosynthetic patterns of 18-OH-deoxycorticosterone (18-OH-DOC) and corticosterone (B) in adrenal tissue of Wistar-rats and two rat strains with salt-dependent hypertension (Dahl-, Sabra-rats) were measured by means of quantitative HPLC-analysis. The relative activity of 18-hydroxylation is expressed as the ratio 18-OH-DOC/18-OH-DOC + B, in the following called Q. Wistar-rats show a constant product ratio Q of 0.40 +/- 0.01. Dahl-rats, however, exhibit typical differences between the two substrains. Adrenal tissue of salt-resistant (Dahl-R) rats produces significantly less 18-OH-DOC than tissue from salt-sensitive (Dahl-S) rats: Q = 0.17 +/- 0.01 (Dahl-R) vs. Q = 0.37 +/- 0.01 (Dahl-S). No such difference was observed between the two Sabra-substrains. This indicates, that other mechanisms than an altered 11 beta-18-hydroxylation are involved in the salt-dependent hypertension of Sabra-rats.

摘要

采用定量高效液相色谱分析法测定了Wistar大鼠以及两种盐依赖性高血压大鼠品系（Dahl大鼠、Sabra大鼠）肾上腺组织中18-羟脱氧皮质酮（18-OH-DOC）和皮质酮（B）的生物合成模式。18-羟化的相对活性以18-OH-DOC/18-OH-DOC + B的比值表示，以下称为Q。Wistar大鼠的产物比值Q恒定为0.40±0.01。然而，Dahl大鼠在两个亚品系之间表现出典型差异。盐抵抗性（Dahl-R）大鼠的肾上腺组织产生的18-OH-DOC明显少于盐敏感性（Dahl-S）大鼠的组织：Q = 0.17±0.01（Dahl-R），而Q = 0.37±0.01（Dahl-S）。在Sabra大鼠的两个亚品系之间未观察到这种差异。这表明，除了11β-18-羟化改变之外，其他机制也参与了Sabra大鼠的盐依赖性高血压。