Romanelli Paulo Roberto, Matos Andressa Maria Rorato Nascimento de, Pinto-Ferreira Fernanda, Caldart Eloiza Teles, Carmo João Lucas Moura do, Santos Nathalia Gomes Dos, Silva Natalia Ribeiro da, Loeffler Barbara Barcellos, Sanches João Fernando Zamariola, Francisquini Luiza Schumacher, Romanelli Micheline Sahyun, Minho Alessandro Pelegrine, Cavalcante Antônio Cezar Rocha, Pierre Elzira Jorge, Sobezak Claudio Cesar, Freire Roberta Lemos, Mitsuka-Breganó Regina, Navarro Italmar Teodorico
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, PR, Brasil.
Universidade Filadélfia - UNIFIL, Londrina, PR, Brasil.
Rev Bras Parasitol Vet. 2021 Apr 26;30(1):e023220. doi: 10.1590/S1984-29612021021. eCollection 2021.
The aim of this study was to evaluate the seroprevalence and factors associated with the presence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep from Paraná state. The detection of antibodies for T. gondii and N. caninum was performed by homemade and commercial indirect ELISA, respectively. Multiple logistic regression analysis was used to verify the factors associated with the seroprevalence. Antibodies anti-T. gondii and anti-N. caninum were observed in 42.7% and in 17.6% of the animals, respectively. The protective factors associated to seropositive were "some level of confinement" (full or semi-extensive confinement) (OR=0.53) for T. gondii and "use of skilled labor" (OR=0.64) for N. caninum. The risk factors were "presence of cats" (OR=1.75) for T. gondii and "feeding of dogs with sheep placental remains" (OR=1.79) for N. caninum. In addition, to presenting a significant and simultaneous seroprevalence for both agents (9.9% of the animals), the results also indicate that deficiencies in management and environmental sanitation, the presence of reservoirs, and types of exploitation enhance the seropositivity. Thus, studies like this might support sanitary programs and public policies for the prevention of T. gondii and N. caninum in the sheep herds of Paraná state.
本研究旨在评估巴拉那州绵羊中抗刚地弓形虫和抗犬新孢子虫抗体的血清流行率及相关因素。分别采用自制和商业间接ELISA法检测刚地弓形虫和犬新孢子虫的抗体。采用多元逻辑回归分析来验证与血清流行率相关的因素。在42.7%的动物中检测到抗刚地弓形虫抗体，在17.6%的动物中检测到抗犬新孢子虫抗体。与血清阳性相关的保护因素，对于刚地弓形虫是“一定程度的圈养”（完全或半粗放圈养）（OR = 0.53），对于犬新孢子虫是“使用熟练劳动力”（OR = 0.64）。风险因素，对于刚地弓形虫是“有猫存在”（OR = 1.75），对于犬新孢子虫是“用羊胎盘残骸喂狗”（OR = 1.79）。此外，两种病原体同时呈现显著的血清流行率（9.9%的动物），结果还表明管理和环境卫生方面的不足、宿主的存在以及养殖类型会增加血清阳性率。因此，这样的研究可能会支持巴拉那州绵羊群预防刚地弓形虫和犬新孢子虫的卫生计划和公共政策。
