土耳其 Klokov 的精油化学成分、抗菌、抗癌和抗氧化作用。

Essential oil chemical composition, antimicrobial, anticancer, and antioxidant effects of Klokov in Turkey.

机构信息

Department of Molecular Biology and Genetics, Faculty of Science and Literature, Balikesir University, 10145Balıkesir, Turkey.

Department of Basic Pharmaceutical Sciences, Division of Analytical Chemistry, Faculty of Pharmacy, İnönü University, 44280Malatya, Turkey.

出版信息

Z Naturforsch C J Biosci. 2021 Jan 19;76(5-6):193-203. doi: 10.1515/znc-2020-0070. Print 2021 May 26.

DOI:10.1515/znc-2020-0070

PMID:33909957

Abstract

In this study, the chemical composition, antimicrobial, antioxidant, and anticancer effects of Klokov oil and its main compound camphor were investigated. The oil was isolated from using hydrodistillation method, analyzed by gas chromatography/mass spectrometry (GC-MS), and 66 compounds were identified. The main component was determined as camphor at 16.6%. The antioxidant properties were identified with the DPPH (2,2'-diphenyl-1-picrylhydrazyl) radical-scavenging method and, 33.39 ± 0.25% DPPH was scavenging in 1000 μg/mL of essential oil. The strong antimicrobial activity was observed against and with MIC values of 125 μg/mL. was more sensitive (28%) against essential oil than other fungi. The cytotoxic effect of oil was analyzed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method. Camphor was effective on human hepatoma cells (Hep3B) at concentrations of 1 mg/mL, 500, 250, and 125 μg/mL, while essential oil of was found to be effective at concentrations of 250 and 125 μg/mL. A reduction in cell proliferation was observed in colon carcinoma cells (HT-29) treated with 500 μg/mL camphor for 48 h. No statistically significant effect was found in Umbilical Vein Endothelial Cells (HUVEC) treated with essential oil and camphor.

摘要

本研究对 Klokov 油及其主要成分樟脑的化学成分、抗菌、抗氧化和抗癌作用进行了研究。该油是通过水蒸馏法从 Klokov 中分离出来的，用气相色谱/质谱联用仪（GC-MS）进行分析，鉴定出 66 种化合物。主要成分确定为樟脑，含量为 16.6%。抗氧化性能采用 DPPH（2,2'-二苯基-1-苦基肼）自由基清除法进行鉴定，在 1000μg/mL 精油中，DPPH 的清除率为 33.39±0.25%。对和表现出较强的抗菌活性，MIC 值均为 125μg/mL。与其他真菌相比，对精油更为敏感（28%）。用 MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法分析油的细胞毒性。樟脑对人肝癌细胞（Hep3B）在 1mg/mL、500、250 和 125μg/mL 浓度下有效，而精油在 250 和 125μg/mL 浓度下有效。用 500μg/mL 樟脑处理结肠癌细胞（HT-29）48 小时后，观察到细胞增殖减少。用精油和樟脑处理脐静脉内皮细胞（HUVEC）未发现有统计学意义的影响。