Institute of Food Nutrition and Health, Eidgenössische Technische Hochschule Zürich (ETH), Schwerzenbach 8603, Switzerland.
Institute of Food Nutrition and Health, Eidgenössische Technische Hochschule Zürich (ETH), Schwerzenbach 8603, Switzerland.
Cell Rep. 2021 Apr 27;35(4):109023. doi: 10.1016/j.celrep.2021.109023.
To analyze the capacity of white and brown adipose tissue remodeling, we developed two mouse lines to label, quantitatively trace, and ablate white, brown, and brite/beige adipocytes at different ambient temperatures. We show here that the brown adipocytes are recruited first and reach a peak after 1 week of cold stimulation followed by a decline during prolonged cold exposure. On the contrary, brite/beige cell numbers plateau after 3 weeks of cold exposure. At thermoneutrality, brown adipose tissue, in spite of being masked by a white-like morphology, retains its brown-like physiology, as Ucp1 cells can be recovered immediately upon beta3-adrenergic stimulation. We further demonstrate that the recruitment of Ucp1 cells in response to cold is driven by existing adipocytes. In contrast, the regeneration of the interscapular brown adipose tissue following ablation of Ucp1 cells is driven by de novo differentiation.
为了分析白色和棕色脂肪组织重塑的能力，我们开发了两种小鼠品系，用于在不同环境温度下标记、定量追踪和消融白色、棕色和米色/ beige 脂肪细胞。我们在这里表明，棕色脂肪细胞首先被募集，在冷刺激 1 周后达到峰值，然后在长时间的寒冷暴露中下降。相反，米色/ beige 细胞数量在冷暴露 3 周后达到平台期。在体温正常的情况下，棕色脂肪组织尽管被白色样形态所掩盖，但仍然保留着棕色样的生理学特性，因为 Ucp1 细胞可以在β3-肾上腺素能刺激下立即恢复。我们进一步证明，冷刺激下 Ucp1 细胞的募集是由现有脂肪细胞驱动的。相比之下，Ucp1 细胞消融后肩胛间棕色脂肪组织的再生是由新分化驱动的。
