Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Qingdao 266100, China.
Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Bioresour Technol. 2021 Aug;333:125198. doi: 10.1016/j.biortech.2021.125198. Epub 2021 Apr 22.
The effect of aerobic/anoxic duration on the performance, microbial community and enzymatic activity of sequencing batch biofilm reactor (SBBR) were investigated in treating mariculture wastewater. The microbial oxygen uptake rate and nitrifying rate gradually decreased with the aerobic/anoxic duration from 120/210 to 30/300 min, whereas the nitrite reducing rate and nitrate reducing rate had the opposite results. The activities of dehydrogenase, ammonia monooxygenase and nitrite oxidoreductase gradually decreased with the aerobic/anoxic duration from 120/210 to 30/300 min, but the activities of nitrate reductase and nitrite reductase had a gradual increment. The microbial nitrogen removal rates had similar changing trends to their corresponding enzymatic activities at different aerobic/anoxic duration. The variation of aerobic/anoxic duration obviously affected the microbial richness and diversity of SBBR. The co-occurrence, keystone taxa and significant difference of microbial community had some changes with the aerobic/anoxic duration from 120/210 to 30/300 min.
考察了好氧/缺氧时间对序批式生物膜反应器（SBBR）处理海水养殖废水性能、微生物群落和酶活性的影响。随着好氧/缺氧时间从 120/210 分钟变为 30/300 分钟，微生物耗氧速率和硝化速率逐渐降低，而亚硝酸盐还原速率和硝酸盐还原速率则相反。脱氢酶、氨单加氧酶和亚硝酸盐氧化还原酶的活性随着好氧/缺氧时间从 120/210 分钟变为 30/300 分钟而逐渐降低，但硝酸盐还原酶和亚硝酸盐还原酶的活性则逐渐增加。在不同的好氧/缺氧时间下，微生物氮去除率与相应的酶活性具有相似的变化趋势。好氧/缺氧时间的变化明显影响了 SBBR 的微生物丰富度和多样性。随着好氧/缺氧时间从 120/210 分钟变为 30/300 分钟，微生物群落的共生、关键类群和显著差异发生了一些变化。
