Peng Wanhong, Tan Qingyu, Yu Minglan, Wang Ping, Wang Tingting, Yuan Jixiang, Liu Dongmei, Chen Dechao, Huang Chaohua, Tan Youguo, Liu Kezhi, Xiang Bo, Liang Xuemei
Department of Psychiatry, Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Affiliated Hospital of Southwest Medical University, Luzhou, China.
Medical Laboratory Center, Affiliated Hospital of Southwest Medical University, Luzhou, China.
Psychiatry Investig. 2021 May;18(5):385-391. doi: 10.30773/pi.2020.0410. Epub 2021 Apr 29.
Schizophrenia (SCZ) is one of the most common and severe mental disorders. Modified electroconvulsive therapy (MECT) is the most effective therapy for all kinds of SCZ, and the underlying molecular mechanism remains unclear. This study is aim to detect the molecule mechanism by constructing the transcriptome dataset from SCZ patients treated with MECT and health controls (HCs).
Transcriptome sequencing was performed on blood samples of 8 SCZ (BECT: before MECT; AECT: after MECT) and 8 HCs, weighted gene co-expression network analysis (WGCNA) was used to cluster the different expression genes, enrichment and protein-protein interaction (PPI) enrichment analysis were used to detect the related pathways.
Three gene modules (black, blue and turquoise) were significantly associated with MECT, enrichment analysis found that the long-term potentiation pathway was associated with MECT. PPI enrichment p-value of black, blue, turquoise module are 0.00127, <1×10-16 and 1.09×10-13, respectively. At the same time, EP300 is a key node in the PPI for genes in black module, which got from the transcriptome sequencing data.
It is suggested that the long-term potentiation pathways were associated with biological mechanism of MECT.
精神分裂症（SCZ）是最常见且最严重的精神障碍之一。改良电休克治疗（MECT）是治疗各类SCZ最有效的方法，但其潜在分子机制仍不清楚。本研究旨在通过构建接受MECT治疗的SCZ患者和健康对照（HCs）的转录组数据集来检测其分子机制。
对8例SCZ患者（BECT：MECT治疗前；AECT：MECT治疗后）和8例HCs的血液样本进行转录组测序，采用加权基因共表达网络分析（WGCNA）对差异表达基因进行聚类，利用富集分析和蛋白质-蛋白质相互作用（PPI）富集分析检测相关通路。
三个基因模块（黑色、蓝色和绿松石色）与MECT显著相关，富集分析发现长时程增强通路与MECT相关。黑色、蓝色、绿松石色模块的PPI富集p值分别为0.00127、<1×10-16和1.09×10-13。同时，EP300是黑色模块基因PPI中的关键节点，这是从转录组测序数据中获得的。
提示长时程增强通路与MECT的生物学机制相关。
