Maia João M, Amorim Vítor A, Viveiros Duarte, Marques P V S
CAP - Centre for Applied Photonics, INESC TEC, 4150-179, Porto, Portugal.
Departament of Physics and Astronomy, Faculty of Sciences, University of Porto, 4169-007, Porto, Portugal.
Sci Rep. 2021 Apr 28;11(1):9128. doi: 10.1038/s41598-021-88682-x.
A monolithic lab-on-a-chip fabricated by femtosecond laser micromachining capable of label-free biosensing is reported. The device is entirely made of fused silica, and consists of a microdisk resonator integrated inside a microfluidic channel. Whispering gallery modes are excited by the evanescent field of a circular suspended waveguide, also incorporated within the channel. Thermal annealing is performed to decrease the surface roughness of the microstructures to a nanometric scale, thereby reducing intrinsic losses and maximizing the Q-factor. Further, thermally-induced morphing is used to position, with submicrometric precision, the suspended waveguide tangent to the microresonator to enhance the spatial overlap between the evanescent field of both optical modes. With this fabrication method and geometry, the alignment between the waveguide and the resonator is robust and guaranteed at all instances. A maximum sensitivity of 121.5 nm/RIU was obtained at a refractive index of 1.363, whereas near the refractive index range of water-based solutions the sensitivity is 40 nm/RIU. A high Q-factor of 10 is kept throughout the entire measurement range.
报道了一种通过飞秒激光微加工制造的、能够进行无标记生物传感的单片式芯片实验室。该器件完全由熔融石英制成,由集成在微流体通道内的微盘谐振器组成。回音壁模式由同样包含在通道内的圆形悬浮波导的倏逝场激发。进行热退火以将微结构的表面粗糙度降低到纳米尺度,从而减少固有损耗并最大化品质因数。此外,利用热致变形以亚微米精度将悬浮波导定位到与微谐振器相切,以增强两种光学模式的倏逝场之间的空间重叠。采用这种制造方法和几何结构,波导与谐振器之间的对准在所有情况下都很稳健且有保证。在折射率为1.363时获得了121.5 nm/RIU的最大灵敏度,而在水基溶液的折射率范围附近,灵敏度为40 nm/RIU。在整个测量范围内保持10的高品质因数。