Department of Surgery, Seoul National University College of Medicine, Seoul, Republic of Korea.
Department of Surgery, Daejin Medical Center Bundang Jesaeng General Hospital, Sungnam-Si, Republic of Korea.
Sci Rep. 2021 Apr 28;11(1):9212. doi: 10.1038/s41598-021-88653-2.
Peritoneal recurrence (PR) is a major relapse pattern of colorectal cancer (CRC). We investigated whether peritoneal immune cytokines can predict PR. Cytokine concentrations of peritoneal fluid from CRC patients were measured. Patients were grouped according to peritoneal cancer burden (PCB): no tumor cells (≤ pT3), microscopic tumor cells (pT4), or gross tumors (M1c). Cytokine concentrations were compared among the three groups and the associations of those in pT4 patients with and without postoperative PR were assessed. Of the ten cytokines assayed, IL6, IL10, and TGFB1 increased with progression of PCB. Among these, IL10 was a marker of PR in pT4 (N = 61) patients based on ROC curve (p = 0.004). The IL10 cut-off value (14 pg/mL) divided patients into groups with a low (7%, 2 of 29 patients) or high (45%, 16 of 32 patients) 5-year PR (p < 0.001). Multivariable analysis identified high IL10 levels as the independent risk factor for PR. Separation of patients into training and test sets to evaluate the performance of IL10 cut-off model validated this cytokine as a risk factor for PR. Peritoneal IL10 is a prognostic marker of PR in pT4 CRC. Further research is necessary to identify immune response of intraperitoneal CRC growth.
腹膜复发（PR）是结直肠癌（CRC）的主要复发模式。我们研究了腹膜免疫细胞因子是否可以预测 PR。测量了 CRC 患者腹膜液中的细胞因子浓度。根据腹膜癌负担（PCB）将患者分组：无肿瘤细胞（≤pT3）、镜下肿瘤细胞（pT4）或大体肿瘤（M1c）。比较了三组之间的细胞因子浓度，并评估了 pT4 患者中无术后 PR 和有术后 PR 的细胞因子浓度之间的关系。在检测的十种细胞因子中，IL6、IL10 和 TGFB1 随着 PCB 的进展而增加。其中，IL10 是基于 ROC 曲线的 pT4（N=61）患者 PR 的标志物（p=0.004）。IL10 截断值（14pg/mL）将患者分为低（7%，29 例中有 2 例）或高（45%，32 例中有 16 例）5 年 PR 组（p<0.001）。多变量分析确定高 IL10 水平是 PR 的独立危险因素。将患者分为训练集和测试集以评估 IL10 截断模型的性能，验证了该细胞因子是 PR 的危险因素。腹膜 IL10 是 pT4CRC 中 PR 的预后标志物。需要进一步研究以确定腹腔内 CRC 生长的免疫反应。
