Crétin C, Luchetta P, Joly C, Miginiac-Maslow M, Decottignies P, Jacquot J P, Vidal J, Gadal P
Laboratoire de Physiologie Végétale Moléculaire, Unité Associée 1128, Université Paris-Sud, Orsay, France.
Eur J Biochem. 1988 Jun 15;174(3):497-501. doi: 10.1111/j.1432-1033.1988.tb14126.x.
The mechanisms underlying the photoregulation of the synthesis of sorghum leaf malate dehydrogenase (NADP) (EC 1.1.1.82) (NADP-MDH), a key enzyme in C4 photosynthesis, have been investigated. During the greening process a light-dependent increase in enzyme activity took place, accompanied by de novo synthesis of the protein. In vitro translation experiments showed that this chloroplastic protein is synthesized as a precursor (46 kDa) with a 'transit peptide' of about 2.5 kDa. A large increase in NADP-MDH-translatable RNAs was also observed during greening. We describe also the construction and characterization of a cDNA clone for NADP-MDH (pCM18A) in the expression vector lambda gt11. The use of this homologous probe demonstrated a light-dependent mRNA accumulation.
高粱叶片苹果酸脱氢酶（NADP）（EC 1.1.1.82）（NADP-MDH）是C4光合作用中的关键酶，对其光调节合成的潜在机制进行了研究。在绿化过程中，酶活性出现光依赖性增加，并伴随着蛋白质的从头合成。体外翻译实验表明，这种叶绿体蛋白作为前体（46 kDa）合成，带有约2.5 kDa的“转运肽”。在绿化过程中还观察到NADP-MDH可翻译RNA大幅增加。我们还描述了在表达载体λgt11中构建和鉴定NADP-MDH的cDNA克隆（pCM18A）。使用这种同源探针证明了mRNA的光依赖性积累。
