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从巴基斯坦开伯尔-普赫图赫瓦省哈扎拉磷矿储备区分离的植物促生细菌对玉米生长和产量提高的效率

Efficiency of plant growth promoting bacteria for growth and yield enhancement of maize () isolated from rock phosphate reserve area Hazara Khyber Pakhtunkhwa, Pakistan.

作者信息

Zainab Rimsha, Mujtaba Shah Ghulam, Khan Waqar, Mehmood Ayaz, Azad Rashid, Shahzad Khurram, Hussain Shah Zahid, Alghabari Fahad, Sultan Tariq, Chung Gyuhwa

机构信息

Department of Botany, Hazara University Mansehra, Pakistan.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Saudi J Biol Sci. 2021 Apr;28(4):2316-2322. doi: 10.1016/j.sjbs.2021.01.025. Epub 2021 Jan 21.

Abstract

The usage of novel Plant Growth-Promoting Rhizobacteria (PGPR) as bioinoculant is a good opportunity for ecological farming practices to improve soil condition, quality of grain, crops' yield and biodiversity conservation. The purpose of recent research was focused to examine, isolate and characterize PGP bacteria that colonize the rhizosphere for the duration of the maize plant's seedling. For this purpose, 14 samples of soils and roots in the maize rhizosphere were collected from rock phosphate area of Hazara, Pakistan. Forty morphologically natural bacterial colonies have been extracted and tested for their PGP innovations and biocontrol residences and further recognized as plant production advancing rhizobacteria. To find the effective PGPR strains with numerous activities, an aggregate of 150 bacterial colonies were sequestered from different rhizospheric soils of the Hazara Pakistan rock phosphate area. These tested bacterial strains were subjected to biochemical description and in vitro screening for their plant growth-promoting qualities like generation of indole acetic acid (IAA), alkali (NH3), hydrogen cyanide (HCN), siderophores, catalases, proteases and pectinases. All the isolates of rhizobacteria showed IAA producing capacity, as well as found positive for catalase and HCN. The above results suggested that, in addition to biocontrol marketers, PGPR could be used as biofertilizers to substitute agro-chemicals in order to increase crop production. These microorganisms can therefore be further developed and used for greenhouse and discipline packages.

摘要

使用新型植物促生根际细菌(PGPR)作为生物接种剂,是生态农业实践改善土壤状况、提高谷物品质、增加作物产量和保护生物多样性的良好契机。近期研究的目的集中于检测、分离和鉴定在玉米幼苗期定殖于根际的PGP细菌。为此,从巴基斯坦哈扎拉地区的磷矿区域采集了14份玉米根际土壤和根系样本。已提取出40个形态上自然的细菌菌落,并对其PGP创新能力和生物防治特性进行了测试,进一步鉴定为促进植物生长的根际细菌。为了找到具有多种活性的有效PGPR菌株,从巴基斯坦哈扎拉地区磷矿区域不同的根际土壤中分离出总计150个细菌菌落。对这些测试的细菌菌株进行了生化描述,并对其促进植物生长的特性进行了体外筛选,如吲哚乙酸(IAA)、碱(NH3)、氰化氢(HCN)、铁载体、过氧化氢酶、蛋白酶和果胶酶的产生。所有根际细菌分离株均显示出产生IAA的能力,并且过氧化氢酶和HCN检测呈阳性。上述结果表明,除了生物防治因子外,PGPR还可作为生物肥料来替代农用化学品,以提高作物产量。因此,这些微生物可进一步开发并用于温室和田间应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e861/8071923/436620999cac/gr1.jpg

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