Brito Eric Saymom Andrade, Prado Laís Di Paulie Taborda, Araújo Liana Késia Costa, Arnhold Emmanuel, Matos Moema Pacheco Chediak, de Paula Joelma Abadia Marciano, Ramos Luciana Machado, Fonseca-Alves Carlos Eduardo, de Moura Veridiana Maria Brianezi Dignani
Animal Pathology Service, Federal University of Goiás- UFG, Goiânia, Brazil.
Department of Animal Science, Federal University of Goiás UFG, Goiânia, Brazil.
Front Vet Sci. 2021 Apr 12;8:605286. doi: 10.3389/fvets.2021.605286. eCollection 2021.
Prostatic cancer (PC) stands out in terms of its occurrence, pathophysiology, and unfavorable prognostics in humans and dogs. Natural drugs bear an integrative potential for conventional antineoplastic treatments. In this context, the bioproducts of have been empirically used in different parts of Brazil for the integrative treatment of prostate cancer in humans. However, there is no availability of scientific evidence of the antitumor effects of . Therefore, this study aimed to investigate the bioactive compounds in the latex of using the high-resolution mass spectrophotometry (HRMS) and to evaluate its cytotoxic effects on primary canine PC cell cultures. Four fragments of phorbol ester were identified as potential bioactive compounds using the HRMS. With the help of an MTT ([3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide]) assay, two canine prostatic carcinoma cell lines (PC 1 and PC2) showed a decrease in the tumor cell count, with an Inhibitory concentration 50 (IC)of 0.8469 and 0.6068 mg/ml, respectively, for PC1 and PC2. In conclusion, the latex of contains phorbol esters in its composition, and its aqueous solution has a cytotoxic effect on canine metastatic PC cells .
前列腺癌（PC）在人类和犬类的发病率、病理生理学及不良预后方面较为突出。天然药物在传统抗肿瘤治疗中具有综合潜力。在此背景下，[植物名称]的生物制品已在巴西不同地区被经验性地用于人类前列腺癌的综合治疗。然而，关于[植物名称]抗肿瘤作用的科学证据尚不充分。因此，本研究旨在利用高分辨率质谱法（HRMS）研究[植物名称]乳胶中的生物活性化合物，并评估其对犬原发性PC细胞培养物的细胞毒性作用。利用HRMS鉴定出四种佛波酯片段为潜在的生物活性化合物。借助MTT（[3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐]）试验，两种犬前列腺癌细胞系（PC 1和PC2）的肿瘤细胞数量减少，PC1和PC2的半数抑制浓度（IC50）分别为0.8469和0.6068 mg/ml。总之，[植物名称]的乳胶成分中含有佛波酯，其水溶液对犬转移性PC细胞具有细胞毒性作用。
