Dudani A K, Gupta R S, Gupta R
McMaster University, Department of Biochemistry, Hamilton, Ontario, Canada.
FEBS Lett. 1988 Jul 4;234(1):141-4. doi: 10.1016/0014-5793(88)81320-6.
The toxicity of the protein synthesis inhibitor puromycin towards a number of human and Chinese hamster cell lines has been examined. In comparison to cells of human origin, Chinese hamster cells exhibited about 25-fold higher resistance towards puromycin. These differences appeared to be species related as all the cell lines from any one species showed similar sensitivity towards puromycin. The incorporation of [3H]leucine in the hamster cell lines was accordingly found to be more resistant to the inhibitory effects of puromycin as compared to human cells. Studies on the cellular uptake of [3H]puromycin showed that in comparison to human cells, the drug uptake/binding in the hamster cell lines was greatly reduced. However, protein synthesis in the extracts of hamster and human cells showed no significant differences in sensitivity towards puromycin. These results show that the observed species related differences in cellular toxicity to puromycin are due to differences in the cellular uptake/binding of the drug.
已检测了蛋白质合成抑制剂嘌呤霉素对多种人类和中国仓鼠细胞系的毒性。与源自人类的细胞相比，中国仓鼠细胞对嘌呤霉素的抗性高出约25倍。这些差异似乎与物种相关，因为来自任何一个物种的所有细胞系对嘌呤霉素都表现出相似的敏感性。因此，与人类细胞相比，发现仓鼠细胞系中[3H]亮氨酸的掺入对嘌呤霉素的抑制作用更具抗性。对[3H]嘌呤霉素细胞摄取的研究表明，与人类细胞相比，仓鼠细胞系中药物的摄取/结合大大减少。然而，仓鼠和人类细胞提取物中的蛋白质合成对嘌呤霉素的敏感性没有显著差异。这些结果表明，观察到的物种相关的对嘌呤霉素细胞毒性差异是由于药物细胞摄取/结合的差异。
