Department of Pharmaceutical Analysis, Sri Adichunchanagiri College of Pharmacy, Adichunchagiri University, B G Nagara, Nagamangala 571448, India.
Department of Pharmacology, Sri Adichunchanagiri College of Pharmacy, Adichunchagiri University, B G Nagara, Nagamangala 571448, India.
J Chromatogr Sci. 2021 Jun 21;59(7):627-633. doi: 10.1093/chromsci/bmab046.
A simple, precise, rapid and accurate UFLC method has been developed with due validation for the simultaneous estimation of Amlodipine besylate and Celecoxib in rat plasma. The separation has been taken place by C18 Eclipse plus column at 1ml/min flow rate. The mobile phase comprises of 20 mM sodium acetate buffer of pH 4.5 adjusted with glacial acetic acid and methanol (30:70% v/v). The effluents were monitored at 228 nm with a total run time of 15min. The retention time of Amlodipine besylate and celecoxib were found to be 7.69 min and 10.69 min respectively. The extraction of drugs have been achieved by protein precipitation technique with methanol as a solvent. The detection concentration was linear over 60-420 ng/ml for Amlodipine besylate and 600-4200 ng/ml for Celecoxib. Regression equation of Amlodipine besylate and Celecoxib were found to be y = 30.996x + 520.29 & y = 39.722x + 23706 with regression coefficient 0.9944 & 0.9941 respectively using unweighted and weighted linear regression with a weighting factor of 1/x0, 1/x, 1/✓x and 1/x2. The percentage recoveries were found to be 88.52±1.276 to 93.06±2.872 for Amlodipine & 89.40±0.728 to 94.05±0.221 for Celecoxib. This liquid chromatography method was extensively validated for linearity, accuracy precision, and stability studies.
已建立了一种简单、精确、快速和准确的 UFLC 方法，并对其进行了验证，可用于同时测定大鼠血浆中的苯磺酸氨氯地平和塞来昔布。分离是在 C18 Eclipse plus 柱上以 1ml/min 的流速进行的。流动相由 20mM 醋酸钠缓冲液（pH4.5）和甲醇（30:70%v/v）组成。流出物在 228nm 处监测，总运行时间为 15min。氨氯地平和塞来昔布的保留时间分别为 7.69min 和 10.69min。药物的提取是通过甲醇作为溶剂的蛋白质沉淀技术实现的。检测浓度在 60-420ng/ml 范围内对苯磺酸氨氯地平呈线性，在 600-4200ng/ml 范围内对塞来昔布呈线性。用无权重和权重线性回归（权重因子为 1/x0、1/x、1/✓x 和 1/x2），得到苯磺酸氨氯地平和塞来昔布的回归方程分别为 y = 30.996x + 520.29 和 y = 39.722x + 23706，回归系数分别为 0.9944 和 0.9941。氨氯地平的回收率为 88.52±1.276%至 93.06±2.872%，塞来昔布的回收率为 89.40±0.728%至 94.05±0.221%。该液相色谱法已广泛验证了线性、准确度、精密度和稳定性研究。
