利用增强型化学发光免疫分析（LIA）检测尿促性腺激素评估青春期发育。

Urinary gonadotropin measurements by enhanced luminometric assays (LIA) for the evaluation of pubertal development.

机构信息

New Children's Hospital, Pediatric Research Center, University of Helsinki, Helsinki University Hospital, Helsinki, Finland.

Department of Pediatrics, Faculty of Medicine, Dokuz Eylül University, Izmir, Turkey.

出版信息

J Pediatr Endocrinol Metab. 2021 Apr 29;34(7):859-866. doi: 10.1515/jpem-2020-0598. Print 2021 Jul 27.

DOI:10.1515/jpem-2020-0598

PMID:33913305

Abstract

OBJECTIVES

Determination of LH in urine has proved to be a reliable method for evaluation of pubertal development. The human LH assay based on time-resolved immunofluorometric (IFMA) technology (AutoDELFIA, PerkinElmer, Wallac) has been found to be suitable for this purpose thanks to its high sensitivity but other assays have not been evaluated. We have analyzed our data obtained by another potentially sensitive detection technique, enhanced luminometric assay (LIA) with the objective of finding a viable alternative to IFMA since these may not be available in the future.

METHODS

LIA was used to measure LH and FSH in serum and urine samples from 100 healthy subjects of each Tanner stage and both genders, whose pubertal development has been determined.

RESULTS

Urinary gonodotropin concentrations measured by LIA correlated well with Tanner stage [(r=0.93 for girls, r=0.81 for boys; p<0.01 for LH) and (r=0.81 for girls, r=0.73 for boys; p<0.01 for FSH)]. LIA determinations revealed the increase in U-LH concentrations during the transition from Tanner stage 1-2 in both girls and boys (p<0.001), whereas U-FSH and S-LH were able to detect the increase from Tanner stage 1-2 only in boys or girls, respectively (both p<0.001).

CONCLUSIONS

Measurement of urinary gonadotropin concentrations by LIA may be useful for the evaluation of overall pubertal development and also in the detection of transition from prepuberty to puberty.

摘要

目的

尿液中 LH 的测定已被证明是评估青春期发育的可靠方法。基于时间分辨免疫荧光（IFMA）技术（AutoDELFIA，PerkinElmer，Wallac）的人 LH 测定由于其高灵敏度而适用于此目的，但其他测定方法尚未得到评估。我们分析了使用另一种潜在敏感检测技术（增强发光测定法，LIA）获得的数据，目的是找到一种可行的 IFMA 替代方法，因为未来这些方法可能无法获得。

方法

LIA 用于测量 100 名每个 Tanner 阶段的健康受试者的血清和尿液样本中的 LH 和 FSH，这些受试者的青春期发育已经确定。

结果

LIA 测量的尿促性腺激素浓度与 Tanner 阶段密切相关[(女孩 r=0.93，男孩 r=0.81；p<0.01 用于 LH)和（女孩 r=0.81，男孩 r=0.73；p<0.01 用于 FSH)]。LIA 测定显示，在女孩和男孩从 Tanner 阶段 1-2 过渡期间，U-LH 浓度增加（p<0.001），而 U-FSH 和 S-LH 仅能分别在男孩或女孩中检测到从 Tanner 阶段 1-2 的增加（均 p<0.001）。