Demir A, Dunkel L, Stenman U H, Voutilainen R
Children's Hospital, University of Helsinki, Finland.
J Clin Endocrinol Metab. 1995 Apr;80(4):1457-60. doi: 10.1210/jcem.80.4.7714124.
Serum levels of LH and FSH are very low from about 2 yr of age to the onset of puberty, which is heralded by a very sharp increase in LH levels. We studied age-related changes in urinary gonadotropins in a total of 184 boys and girls of various ages. Urinary FSH and LH were measured by ultrasensitive time-resolved immunofluorometric assays. The detection limit was 0.015 IU/L for LH and 0.018 IU/L for FSH. We observed that after an initial drop following the first months of life, urinary LH levels stayed below 0.5 IU/L until age 9 yr in girls and below 1.0 IU/L until age 11 yr in boys, whereas mean urinary FSH levels remained below 3.0 IU/L until age 10 yr in girls and 12 yr in boys. During puberty, mean urinary FSH and LH concentrations increased to about 5 IU/L in boys and 10 IU/L in girls. This corresponds to a 5-fold increase in FSH in both sexes and a 50- to 100-fold increase in LH in boys and girls, respectively. These dynamic changes agree with previous reports regarding serum levels, suggesting that noninvasive urinary gonadotropin measurements can be a viable alternative to serum determinations in the evaluation of gonadotropin secretion during childhood and adolescence.
从大约2岁到青春期开始，血清促黄体生成素（LH）和促卵泡生成素（FSH）水平非常低，而LH水平的急剧升高预示着青春期的开始。我们研究了总共184名不同年龄段男孩和女孩尿促性腺激素的年龄相关变化。采用超灵敏时间分辨免疫荧光分析法测定尿FSH和LH。LH的检测限为0.015 IU/L，FSH的检测限为0.018 IU/L。我们观察到，在出生后的头几个月出现初始下降后，女孩尿LH水平在9岁前一直低于0.5 IU/L，男孩在11岁前一直低于1.0 IU/L，而女孩尿FSH平均水平在10岁前、男孩在12岁前一直低于3.0 IU/L。在青春期，男孩尿FSH和LH平均浓度增加到约5 IU/L，女孩增加到约10 IU/L。这相当于两性FSH均增加5倍，男孩和女孩LH分别增加50至100倍。这些动态变化与先前关于血清水平的报告一致，表明在评估儿童和青少年促性腺激素分泌时，非侵入性尿促性腺激素测量可以作为血清测定的可行替代方法。
