Betancourt Diana M, Llana Mariángeles Noto, Sarnacki Sebastián H, Cerquetti M Cristina, Monzalve Liliana Salazar, Pustovrh María C, Giacomodonato Mónica N
Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM-UBA-CONICET), Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, UBA, Buenos Aires, Argentina.
Departamento de Morfología, Facultad de Salud, Universidad Del Valle, Cali, Colombia.
Placenta. 2021 Jun;109:11-18. doi: 10.1016/j.placenta.2021.04.004. Epub 2021 Apr 16.
Salmonella foodborne disease during pregnancy causes a significant fetal loss in domestic livestock and preterm birth, chorioamnionitis and miscarriage in humans. These complications could be associated with alterations in placental structure. This study was aimed to determine how a low dose of Salmonella Enteritidis during late gestation affects placental histomorphometric in mice.
We used a self-limiting enterocolitis murine model. BALB/c pregnant animals received a low dose of Salmonella Enteritidis (3-4 x 10 CFU/mouse) on gestational day (GD) 15. At day 3 post infection bacterial loads, serum cytokines expression and placental histomorphometrics parameters were analyzed.
We found that a sub-lethal infection with Salmonella induced a significant drop in fetal weight -to-placental weight-ratio and an increase in the placental coefficient. After bacterial inoculation maternal organs were colonized, inducing placental morphometric alterations, including increased placental thickness, reduced surface area, and diminished major and minor diameters. Also, foci of necrosis accompanied by acute leukocyte infiltration in decidual zone, reduction of vascular spaces and vascular congestion in labyrinth zone, were also evident in placentas from infected females on GD 18. Our data shows that placentas from infected mothers are phenotypically different from control ones. Furthermore, expression of IFN-gamma and IL-6 was up regulated in response to Salmonella in maternal serum.
Our findings demonstrate that a low dose of Salmonella during late gestation alters the placental morphometry leading to negative consequences on pregnancy outcome such as significant reduction in fetal body weight.
孕期沙门氏菌食源性疾病会导致家畜大量胎儿死亡，以及人类早产、绒毛膜羊膜炎和流产。这些并发症可能与胎盘结构改变有关。本研究旨在确定妊娠晚期低剂量肠炎沙门氏菌如何影响小鼠胎盘组织形态计量学。
我们使用了一种自限性小肠结肠炎小鼠模型。BALB/c 孕鼠在妊娠第 15 天接受低剂量肠炎沙门氏菌（3 - 4×10 CFU/小鼠）。在感染后第 3 天，分析细菌载量、血清细胞因子表达和胎盘组织形态计量学参数。
我们发现，沙门氏菌的亚致死感染导致胎儿体重与胎盘重量之比显著下降，胎盘系数增加。细菌接种后，母体器官被定植，导致胎盘形态计量学改变，包括胎盘厚度增加、表面积减小以及长径和短径减小。此外，在妊娠第 18 天感染母鼠的胎盘中，蜕膜区出现坏死灶并伴有急性白细胞浸润，迷路区血管间隙减少和血管充血也很明显。我们的数据表明，感染母亲的胎盘在表型上与对照胎盘不同。此外，母体血清中 IFN-γ 和 IL-6 的表达因沙门氏菌而上调。
我们的研究结果表明，妊娠晚期低剂量沙门氏菌会改变胎盘形态计量学，对妊娠结局产生负面影响，如胎儿体重显著降低。
