Department of Otolaryngology-Head and Neck Surgery, School of Medicine, Catholic University of Daegu, Daegu 42472, Korea.
Molecules. 2021 Apr 1;26(7):2002. doi: 10.3390/molecules26072002.
Korean Red ginseng (KRG), commonly used in traditional medicine, has anti-inflammatory, anti- oxidative, and anti-tumorigenic properties. Asian sand dust (ASD) is known to aggravate upper and lower airway inflammatory responses. BEAS-2B cells were exposed to ASD with or without KRG or ginsenoside Rg3. Mucin 5AC (MUC5AC), MUC5B, and MUC8 mRNA and protein expression levels were determined using quantitative RT-PCR and enzyme-linked immunosorbent assay. Nuclear factor kappa B (NF-κB), activator protein 1, and mitogen-activated protein kinase expression and activity were determined using western blot analysis. ASD induced MUC5AC, MUC5B, and MUC8 mRNA and protein expression in BEAS-2B cells, which was significantly inhibited by KRG and Rg3. Although ASD-induced mucin expression was associated with NF-κB and p38 mitogen-activated protein kinase (MAPK) activity, KRG and Rg3 significantly suppressed only ASD-induced NF-κB expression and activity. KRG and Rg3 inhibited ASD-induced mucin gene expression and protein production from bronchial epithelial cells. These results suggest that KRG and Rg3 have potential for treating mucus-producing airway inflammatory diseases.
高丽红参（KRG）在传统医学中被广泛应用，具有抗炎、抗氧化和抗肿瘤特性。已知亚洲沙尘（ASD）会加重上呼吸道和下呼吸道的炎症反应。本研究将 BEAS-2B 细胞暴露于含有或不含有 KRG 或人参皂苷 Rg3 的 ASD 中。通过定量 RT-PCR 和酶联免疫吸附试验测定粘蛋白 5AC（MUC5AC）、MUC5B 和 MUC8 mRNA 和蛋白表达水平。通过 Western blot 分析测定核因子 kappa B（NF-κB）、激活蛋白 1 和丝裂原活化蛋白激酶表达和活性。ASD 诱导 BEAS-2B 细胞中 MUC5AC、MUC5B 和 MUC8 mRNA 和蛋白表达，KRG 和 Rg3 显著抑制其表达。虽然 ASD 诱导的粘蛋白表达与 NF-κB 和 p38 丝裂原活化蛋白激酶（MAPK）活性有关，但 KRG 和 Rg3 仅显著抑制 ASD 诱导的 NF-κB 表达和活性。KRG 和 Rg3 抑制了支气管上皮细胞中 ASD 诱导的粘蛋白基因表达和蛋白产生。这些结果表明，KRG 和 Rg3 具有治疗粘液产生性气道炎症性疾病的潜力。
