Dąbrowska Joanna, Karamon Jacek, Kochanowski Maciej, Sroka Jacek, Cencek Tomasz
Department of Parasitology, National Veterinary Research Institute, Partyzantow Avenue 57, 24-100 Puławy, Poland.
Pathogens. 2021 Apr 8;10(4):441. doi: 10.3390/pathogens10040441.
The prevalence of bovine infection has decreased almost to zero in most European countries, such as Poland, which has been -free since 1997. However, trichomonosis is a notifiable disease and there is a duty to examine samples from cattle. In this study, we present an unexpected cross-reaction with -like DNA in a specimen from a bull. The bovine sample was submitted to the Department of Parasitology National Veterinary Research Institute in Pulawy (NVRI) for confirmatory testing after having been examined at the Regional Veterinary Laboratory, during a routine diagnosis. Positive results from microscopic observation and cultures were confirmed. Noteworthily, parasites grew on Diamond's medium only after seven days of incubation, while optimal growth of trichomonads is generally observed after two to four days for this medium. Moreover, by using PCR we obtained positive results for the presence of . However, sequencing of the amplification product revealed 99.62% identity with sp. Our data suggest that false-positive results may occur in commonly used PCR tests. Thus, unexpected results should be carefully interpreted.
在大多数欧洲国家，如自1997年起就无牛感染的波兰，牛感染的流行率几乎已降至零。然而，毛滴虫病是一种应报告的疾病，有义务对牛的样本进行检测。在本研究中，我们在一头公牛的样本中发现了与类似DNA的意外交叉反应。该牛样本在地区兽医实验室进行常规诊断检查后，被送往普拉维的国家兽医研究所寄生虫学系进行确认检测。显微镜观察和培养的阳性结果得到了证实。值得注意的是，寄生虫仅在培养七天后才在钻石培养基上生长，而对于这种培养基，毛滴虫的最佳生长通常在两到四天后观察到。此外，通过使用聚合酶链反应（PCR），我们获得了存在的阳性结果。然而，扩增产物的测序显示与种的同一性为99.62%。我们的数据表明，在常用的PCR检测中可能会出现假阳性结果。因此，对于意外结果应仔细解读。
