Rodrigues Ana M, Langer Swen, Carrasquinho Isabel, Bergström Ed, Larson Tony, Thomas-Oates Jane, António Carla
Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Av. da República, 2780-157 Oeiras, Portugal.
Centre of Excellence in Mass Spectrometry, University of York, Heslington, York YO10 5DD, UK.
Metabolites. 2021 Apr 8;11(4):227. doi: 10.3390/metabo11040227.
The pinewood nematode (PWN) is the causal agent of pine wilt disease, a pathology that affects conifer forests, mainly spp. PWN infection can induce the expression of phytohormone-related genes; however, changes at the early phytohormone level have not yet been explored. Phytohormones are low-abundance metabolites, and thus, difficult to quantify. Moreover, most methodologies focus mainly on Arabidopsis or crop species. This work aimed to validate a fast (run time 6.6 min) liquid chromatography-triple quadrupole tandem mass spectrometry (LC-QqQ-MS/MS) analytical method to quantify 14 phytohormones in stem tissues. This method was further applied to evaluate, for the first time, early phytohormone changes in susceptible and resistant phenotypes of 24, 48 and 72 h after inoculation (HAI) with PWN. A significant increase in salicylic acid (SA, 48 and 72 HAI) and jasmonic acid methyl ester (JA-ME, 72 HAI) was observed in susceptible phenotypes. Results indicate that the higher susceptibility of to PWN infection might result from an inefficient trigger of hypersensitive responses, with the involvement of JA and SA pathways. This work provides an important update in forest research, and adds to the current knowledge of spp. defence responses to PWN infection.
松材线虫（PWN）是松材线虫病的病原体，该病主要影响针叶林，尤其是 种。PWN 感染可诱导植物激素相关基因的表达；然而，早期植物激素水平的变化尚未得到研究。植物激素是低丰度代谢产物，因此难以定量。此外，大多数方法主要集中在拟南芥或农作物物种上。这项工作旨在验证一种快速（运行时间 6.6 分钟）的液相色谱 - 三重四极杆串联质谱（LC-QqQ-MS/MS）分析方法，用于定量 茎组织中的 14 种植物激素。该方法进一步应用于首次评估接种 PWN 后 24、48 和 72 小时（HAI）易感和抗性表型中早期植物激素的变化。在易感表型中观察到水杨酸（SA，48 和 72 HAI）和茉莉酸甲酯（JA-ME，72 HAI）显著增加。结果表明， 对 PWN 感染的较高易感性可能是由于过敏反应触发效率低下，涉及 JA 和 SA 途径。这项工作为森林研究提供了重要的更新，并增加了目前对 种对 PWN 感染防御反应的认识。
