Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Micro-analytical Lab., Applied Organic Chemistry Department, National Research Center, Dokki, Cairo 12622, Egypt.
Molecules. 2021 Apr 2;26(7):2039. doi: 10.3390/molecules26072039.
The reactions of ketotifen fumarate (KT) with 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) and 7,7,8,8-tetracyanoquinodimethane (TCNQ) as π acceptors to form charge transfer (CT) complexes were evaluated in this study. Experimental and theoretical approaches, including density function theory (DFT), were used to obtain the comprehensive, reliable, and accurate structure elucidation of the developed CT complexes. The CT complexes (KT-DDQ and KT-TCNQ) were monitored at 485 and 843 nm, respectively, and the calibration curve ranged from 10 to 100 ppm for KT-DDQ and 2.5 to 40 ppm for KT-TCNQ. The spectrophotometric methods were validated for the determination of KT, and the stability of the CT complexes was assessed by studying the corresponding spectroscopic physical parameters. The molar ratio of KT:DDQ and KT:TCNQ was estimated at 1:1 using Job's method, which was compatible with the results obtained using the Benesi-Hildebrand equation. Using these complexes, the quantitative determination of KT in its dosage form was successful.
本研究评估了富马酸酮替芬（KT）与 2,3-二氯-5,6-二氰对苯醌（DDQ）和 7,7,8,8-四氰基对醌二甲烷（TCNQ）作为π受体形成电荷转移（CT）配合物的反应。采用实验和理论方法（包括密度泛函理论（DFT）），对所开发的 CT 配合物进行了全面、可靠和准确的结构阐明。CT 配合物（KT-DDQ 和 KT-TCNQ）分别在 485nm 和 843nm 处监测，KT-DDQ 的校准曲线范围为 10-100ppm，KT-TCNQ 的校准曲线范围为 2.5-40ppm。分光光度法用于测定 KT，通过研究相应的光谱物理参数评估 CT 配合物的稳定性。使用 Job 法估计 KT:DDQ 和 KT:TCNQ 的摩尔比为 1:1，这与使用 Benesi-Hildebrand 方程获得的结果一致。使用这些配合物，成功地对其制剂中的 KT 进行了定量测定。
