Rauch P, Fukal L, Brezina P, Kás J
Institute of Chemical Technology, Department of Biochemistry and Microbiology, Prague, Czechoslovakia.
J Assoc Off Anal Chem. 1988 May-Jun;71(3):491-3.
Cross-reactions and resulting nonspecific binding of substances with structures resembling aflatoxins (derivatives of coumarin, and cinnamonic and benzoic acids, etc.) were investigated. The concentrations of these substances causing erroneously high or false positive values in radioimmunoassay were determined. One microgram aflatoxin B1/kg sample may be simulated by the occurrence of 5 g coumarin, 10 g caffeic acid, 16 g chlorogenic acid, or 15 g vanillin/kg fodder or food sample.
研究了与黄曲霉毒素结构相似的物质（香豆素衍生物、肉桂酸和苯甲酸等）的交叉反应及由此产生的非特异性结合。测定了这些物质在放射免疫分析中导致错误高值或假阳性值的浓度。每千克饲料或食品样品中出现5微克香豆素、10微克咖啡酸、16微克绿原酸或15微克香草醛时，可能会模拟出每千克样品含1微克黄曲霉毒素B1的情况。
