Bushway R J, Perkins L B
University of Maine, Department of Food Science, Orono 04469.
J Assoc Off Anal Chem. 1988 May-Jun;71(3):617-8.
A nonaqueous reverse-phase liquid chromatographic (LC) method has been developed to determine elemental sulfur in pesticide formulations. Samples were extracted in 50 mL of stabilized tetrahydrofuran (THF) by gentle swirling while sonicating for 1 min. A 5 microL aliquot was injected into the LC instrument equipped with a Vydac 218 TP 54 column. The mobile phase was methanol-acetonitrile-stabilized tetrahydrofuran (58.5 + 40 + 1.5). Sulfur was monitored at 280 nm. Retention time was approximately 5 min with total analysis time of 7 min. For 6 different products analyzed 12 times each, the coefficients of variations were all less than 3.5%. Purity of each sulfur peak was checked by using a photodiode array detector in the spectrum and absorbance ratio modes. No impurities were observed at the monitoring wavelength.
已开发出一种非水反相液相色谱（LC）方法来测定农药制剂中的元素硫。通过轻轻涡旋并超声处理1分钟，将样品在50 mL稳定的四氢呋喃（THF）中萃取。取5 μL等分试样注入配备Vydac 218 TP 54柱的LC仪器中。流动相为甲醇 - 乙腈 - 稳定的四氢呋喃（58.5 + 40 + 1.5）。在280 nm处监测硫。保留时间约为5分钟，总分析时间为7分钟。对于分析的6种不同产品，每种产品分析12次，变异系数均小于3.5%。使用光电二极管阵列检测器在光谱和吸光度比模式下检查每个硫峰的纯度。在监测波长处未观察到杂质。
