Acosta-Dacal Andrea, Rial-Berriel Cristian, Díaz-Díaz Ricardo, Bernal-Suárez María Del Mar, Zumbado Manuel, Henríquez-Hernández Luis Alberto, Luzardo Octavio P
Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s/n, 35016 Las Palmas de Gran Canaria, Spain.
Department of Environmental Analysis, Technological Institute of the Canary Islands, C/Los Cactus No 68, Polígono Industrial de Arinaga, Agüimes, 35118 Las Palmas, Spain.
Toxics. 2021 Apr 10;9(4):83. doi: 10.3390/toxics9040083.
A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction was validated for the extraction of seven coumarin anticoagulant rodenticides (ARs) and 36 pharmaceutical active compounds (PhACs) residues in soil samples using liquid chromatography tandem mass spectrometry (LC-MS/MS). The aim of this work was to develop a method for the monitoring of these compounds in agricultural lands as well as in forensic applications for the determination of ARs poisoning. As far as is known, this is the first time that a QuEChERS-based method is used for the extraction of ARs in soil, as well as on such a quantity of PhACs. A matrix effect study was carried out on samples of soil devoted to agriculture in the midland area of the Canary Islands (clay loam type). It was in house validated (accuracy, precision, and linearity) at seven spiked levels between 0.5 and 50 ng g. The limits of quantification (LOQ) ranged between 0.5 and 50.0 ng g and the limits of detection (LOD) ranged from 0.024 to 6.25 ng g. The method was then successfully used for both the determination of the target analytes in the soils from the agricultural plots that had been irrigated with regenerated water, and in the soil collected from underneath wild bird carcasses (which had been the subject of forensic investigation).
采用液相色谱串联质谱法（LC-MS/MS），对改良的QuEChERS（快速、简便、廉价、高效、耐用且安全）萃取法进行了验证，用于萃取土壤样品中的七种香豆素类抗凝血灭鼠剂（ARs）和36种药物活性化合物（PhACs）残留。这项工作的目的是开发一种方法，用于监测农田中这些化合物，以及在法医应用中测定ARs中毒情况。据所知，这是首次将基于QuEChERS的方法用于土壤中ARs的萃取，以及如此数量的PhACs的萃取。对加那利群岛中部地区用于农业的土壤样本（粘壤土类型）进行了基质效应研究。该方法在0.5至50 ng g的七个加标水平下进行了内部验证（准确度、精密度和线性）。定量限（LOQ）在0.5至50.0 ng g之间，检测限（LOD）在0.024至6.25 ng g之间。然后，该方法成功用于测定用再生水灌溉的农田土壤以及从野生鸟类尸体下方采集的土壤（该尸体是法医调查对象）中的目标分析物。
