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外膜囊泡的产生代表了一种将毒力因子 CagA、VacA 和 UreA 递送入人类胃腺癌 (AGS) 细胞的方法。

Outer Membrane Vesicle Production by Represents an Approach for the Delivery of Virulence Factors CagA, VacA and UreA into Human Gastric Adenocarcinoma (AGS) Cells.

机构信息

Institute of Molecular Medicine, National Tsing Hua University, Hsinchu 30013, Taiwan.

Division of Gastroenterology, Department of Internal Medicine, Kaoshiung Medical University Hospital, Kaoshiung Medical University, Kaoshiung 80756, Taiwan.

出版信息

Int J Mol Sci. 2021 Apr 11;22(8):3942. doi: 10.3390/ijms22083942.

DOI:10.3390/ijms22083942
PMID:33920443
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8069053/
Abstract

infection is the etiology of several gastric-related diseases including gastric cancer. Cytotoxin associated gene A (CagA), vacuolating cytotoxin A (VacA) and α-subunit of urease (UreA) are three major virulence factors of , and each of them has a distinct entry pathway and pathogenic mechanism during bacterial infection. can shed outer membrane vesicles (OMVs). Therefore, it would be interesting to explore the production kinetics of OMVs and its connection with the entry of key virulence factors into host cells. Here, we isolated OMVs from 26,695 strain and characterized their properties and interaction kinetics with human gastric adenocarcinoma (AGS) cells. We found that the generation of OMVs and the presence of CagA, VacA and UreA in OMVs were a lasting event throughout different phases of bacterial growth. OMVs entered AGS cells mainly through macropinocytosis/phagocytosis. Furthermore, CagA, VacA and UreA could enter AGS cells via OMVs and the treatment with OMVs would cause cell death. Comparison of 26,695 and clinical strains suggested that the production and characteristics of OMVs are not only limited to laboratory strains commonly in use, but a general phenomenon to most strains.

摘要

感染是包括胃癌在内的几种胃部相关疾病的病因。细胞毒素相关基因 A（CagA）、空泡细胞毒素 A（VacA）和脲酶α亚单位（UreA）是幽门螺杆菌的三个主要毒力因子，它们在细菌感染过程中都有独特的进入途径和致病机制。幽门螺杆菌可以脱落外膜囊泡（OMVs）。因此，探索幽门螺杆菌 OMVs 的产生动力学及其与关键毒力因子进入宿主细胞的关系将是一件有趣的事情。在这里，我们从 26,695 株中分离出 OMVs，并对其特性及其与人类胃腺癌（AGS）细胞的相互作用动力学进行了表征。我们发现，OMVs 的产生以及 CagA、VacA 和 UreA 存在于 OMVs 中是细菌生长不同阶段的持续事件。OMVs 主要通过巨胞饮/吞噬作用进入 AGS 细胞。此外，CagA、VacA 和 UreA 可以通过 OMVs 进入 AGS 细胞，并且 OMVs 的处理会导致细胞死亡。与 26,695 株和临床株的比较表明，OMVs 的产生和特性不仅限于常用的实验室菌株，而是大多数 菌株的普遍现象。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc9/8069053/44aeb37ce40f/ijms-22-03942-g006.jpg
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3
OMV Vaccines and the Role of TLR Agonists in Immune Response.OMV 疫苗和 TLR 激动剂在免疫应答中的作用。
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Molecules. 2025 Feb 4;30(3):687. doi: 10.3390/molecules30030687.
4
Extracellular vesicles in Helicobacter pylori-mediated diseases: mechanisms and therapeutic potential.幽门螺杆菌介导的疾病中的细胞外囊泡：机制与治疗潜力
Cell Commun Signal. 2025 Feb 11;23(1):79. doi: 10.1186/s12964-025-02074-6.
5
Opportunities for Eradication beyond Conventional Antibiotics.超越传统抗生素的根除机会。
Microorganisms. 2024 Sep 30;12(10):1986. doi: 10.3390/microorganisms12101986.
6
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Int J Biol Sci. 2024 Jul 15;20(10):4029-4043. doi: 10.7150/ijbs.94156. eCollection 2024.
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J Extracell Biol. 2023 Apr 25;2(5):e84. doi: 10.1002/jex2.84. eCollection 2023 May.
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Int J Mol Sci. 2020 Jun 21;21(12):4416. doi: 10.3390/ijms21124416.
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Sci Rep. 2017 Nov 6;7(1):14526. doi: 10.1038/s41598-017-15204-z.
10
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PLoS Pathog. 2017 Feb 3;13(2):e1006159. doi: 10.1371/journal.ppat.1006159. eCollection 2017 Feb.